水产学报2026，Vol.50Issue(5)：226-238,13.DOI:10.11964/jfc.20260215379
中华绒螯蟹对10种新型蛋白源粗蛋白及氨基酸表观消化率的比较
Comparative study on apparent digestibility coefficients of crude protein and amino acids from ten novel protein sources in Chinese mitten crab(Eriocheir sinensis)
摘要
Abstract
The global shortage and rising cost of fish meal have driven growing interest in novel protein sources for aquafeed.Advanced processing technologies,including enzymatic hydrolysis,fermentation,extrusion,and hydrolysis,have enabled the production of various deeply processed protein ingredients;however,their digestive utilization in aquatic animals remains insufficiently characterized.As a commercially important crustacean,Chinese mitten crab(Eriocheir sinensis)possesses a digestive physiology that differs fundamentally from that of fish,and the direct application of fish-derived digestibility data to crab feed formulation can lead to substantial errors.However,systematic digestibility data for novel protein sources in this spe-cies remain scarce,and whether crude protein apparent digestibility coefficients(ADCs)can reliably predict amino acid avail-ability in highly processed ingredients has not been empirically examined.This study aimed to evaluate the ADCs of ten novel protein ingredients in Chinese mitten crab and to investigate the discrepancy between crude protein and amino acid digestibility across different processing technologies.Ten commercial protein ingredients were tested against fish meal(FM)as the control,including enzymatically hydrolyzed chicken meal(EHCM),feather meal(EHFM),full-fat soybean(EHFS),and soy protein(EHSP);fermented cottonseed meal(FCM)and soybean meal(FSM);extruded soybean meal(ESM)and feather meal(EFM);hydrolyzed feather meal(HFM)and hydrolyzed processed fish meal(HF).Male crabs[initial body weight(125.0±6.1)g]were assigned to 12 groups(3 replicates,6 crabs each).Using yttrium oxide(Y2O3,0.1%)as an inert marker,ADCs of dry mat-ter,crude protein,crude lipid,and 17 amino acids were determined via the 70%basal diet+30%test ingredient substitution method,with fecal collection conducted over 80 days.Ingredient type and processing technology significantly affected all nutri-ent ADCs(P<0.05).Crude protein ADCs ranged from 62.95%(ESM)to 92.80%(EHCM).EHCM and EFM(90.77%)were comparable to FM(88.46%)(P>0.05),while ESM,HFM(65.28%),EHFS(65.11%),and HF(69.22%)were significantly lower(P<0.05).Notably,amino acid ADCs did not consistently align with crude protein ADCs.Despite its lowest crude pro-tein ADC,ESM maintained ADCs above 90%for methionine(94.35%),histidine(95.08%),lysine(96.78%),and alanine(93.69%).Principal component analysis of essential amino acid digestibility explained 86.4%of total variance,and PC1 scores were significantly correlated with the digestibility discrepancy index(DDI=crude protein ADC-mean essential amino acid ADC)(r=0.796,P=0.003).EHCM and FCM exhibited the largest positive DDI values(+30.4%and+27.0%),indicating that non-protein nitrogen substantially inflated crude protein ADC relative to actual amino acid utilization.Conversely,ESM showed the largest negative DDI(-21.8%),demonstrating that crude protein ADC underestimated its amino acid utilization potential.In conclusion,EHCM and EFM are high-quality alternative protein sources for Chinese mitten crab with protein digestibility comparable to fish meal.This study reveals that crude protein ADC is an unreliable predictor of amino acid avail-ability in deeply processed ingredients.It is recommended that amino acid-based digestibility data be incorporated into feed for-mulation systems to correct evaluation biases introduced by processing technologies.关键词
中华绒螯蟹/新型蛋白源/表观消化率/氨基酸消化率Key words
Eriocheir sinensis/novel protein sources/apparent digestibility/amino acid digestibility分类
农业科技引用本文复制引用
王松,袁栋昊,曹子淇,张连杰,王晓丹,陈立侨,李二超..中华绒螯蟹对10种新型蛋白源粗蛋白及氨基酸表观消化率的比较[J].水产学报,2026,50(5):226-238,13.基金项目
国家重点研发计划(2023YFD2402000) （2023YFD2402000）
上海市产业技术体系(202504) （202504）
国家现代农业产业技术体系(CARS-48) National Key Research and Development Program of China(2023YFD2402000) （CARS-48）
Agriculture Research Sys-tem of Shanghai,China(202504) （202504）
Agriculture Research System of China of MOF and MARA(CARS-48) （CARS-48）
