四川医学2026，Vol.47Issue(3)：272-278,7.DOI:10.16252/j.cnki.issn1004-0501-2026.03.006
LncRNA SOX2-OT靶向miR-186-5p/ZEB1轴对黑色素瘤细胞增殖、迁移和侵袭的影响
Effects of LncRNA SOX2-OT on Proliferation,Migration,and Invasion of Melanoma Cells by Targeting the miR-186-5p/ZEB1 Axis
摘要
Abstract
Objective To discuss effects of long non-coding small nucleolar RNA SOX2 overlapping transcript(LncRNA SOX2-OT)on melanoma(MM)cells by targeting microRNA-186-5p(miR-186-5p)/zinc finger E-box binding homeobox protein 1(ZEB1)axis.Methods Normal human skin melanocyte line PIG1 and human MM cell lines A375,M14,and A2058 were cul-tured in vitro,and the LncRNA SOX2-OT,miR-186-5p,and ZEB1 mRNA in each cell were detected.A2058 cells were separated into Control group,si-NC group,si-SOX2-OT group,si-SOX2-OT+inhibitor-NC group,and si-SOX2-OT+miR-186-5p inhibitor group.The LncRNA SOX2-OT,miR-186-5p,ZEB1 mRNA(RT-qPCR method),cell viability(CCK-8 assay kit),proliferation ac-tivity(clone formation assay),migration(scratch assay),invasion ability(Transwell chamber assay),ZEB1,E-cadherin,TGF-β1,and α-SMA proteins were detected by Western blot.Targeting relationships between miR-186-5p and LncRNA SOX2-OT,ZEB1 were validated.Results Compared with control group and si-NC group,si-SOX2-OT group showed a decrease in LncRNA SOX2-OT and ZEB1 mRNAs,OD450 value,cell clone number,scratch healing rate,cell invasion number,and ZEB1,TGF-β1,andα-SMA proteins,as well as an increase in miR-186-5p and E-cadherin protein(P＜0.05).Compared with si-SOX2-OT+inhibi-tor-NC group,si-SOX2-OT+miR-186-5p inhibitor group showed an increase in ZEB1 mRNA,OD450 value,cell clone number,scratch healing rate,cell invasion number,and ZEB1,TGF-β1,and α-SMA proteins,and a decrease in miR-186-5p and Ecadherin protein(P＜0.05).After transfection with miR-186-5p mimic,the luciferase activity of LncRNA SOX2-OT-WT and ZEB1-WT de-creased(P＜0.05).The nude mouse transplantation experiment showed that knocking down SOX2-OT could increase the miR-186-5p and E-cadherin proteins in transplanted tumors,reduce ZEB1 protein,and inhibit the growth of transplanted tumors.Inhibiting miR-186-5p could alleviate the inhibitory effect of knocking down SOX2-OT on the growth of transplanted tumors(P＜0.05).Conclusion LncRNA SOX2-OT is abnormally overexpressed in MM cells.Knocking down LncRNA SOX2-OT could target upregulation of miR-186-5p,inhibit ZEB1,and thus suppress the malignant biological behaviors of MM cells.关键词
长链非编码RNA SOX2重叠转录本/微小RNA-186-5p/锌指E盒结合同源框1轴/黑色素瘤细胞/恶性生物学行为Key words
long non-coding RNA SOX2 overlapping transcript/microRNA-186-5p/zinc finger E-box binding homeobox protein 1/melanoma cells/malignant biological behaviors分类
医药卫生引用本文复制引用
邱文娜,张龙军,冯金,刘波..LncRNA SOX2-OT靶向miR-186-5p/ZEB1轴对黑色素瘤细胞增殖、迁移和侵袭的影响[J].四川医学,2026,47(3):272-278,7.基金项目
河北省卫生健康委员会项目资助(编号:20251413) （编号:20251413）
