四川医学2026，Vol.47Issue(3)：279-286,8.DOI:10.16252/j.cnki.issn1004-0501-2026.03.007
LncRNA KCNQ1OT1调节miR-20a-5p/PTEN轴对OGD/R诱导的海马神经元损伤的影响
Effect of Long Non-Coding RNA KCNQ1OT1 on Oxygen-Glucose Deprivation/Reoxygenation(OGD/R)-Induced Hippocampal Neuronal Damage by Regulating miR-20a-5p/PTEN Axis
摘要
Abstract
Objective To investigate the effect of long non-coding RNA(LncRNA)KCNQ1OT1 on oxygen-glucose dep-rivation/reoxygenation(OGD/R)-induced hippocampal neuronal cell damage by regulating microRNA(miR)-20a-5p/phosphatase and tensin homologue deleted on chromosome ten(PTEN)axis.Methods HT22 cells were assigned into control group,OGD/R group,si-NC,si-KCN,miR-20a-5p mimic-NC,OGD/R+miR-20a-5p mimic,si-KCN+miR inhibitor-NC group,and si-KCN+miR-20a-5p inhibitor group.MTT assay was used to detect the viability of HT22 cells.Reagent kits were used to detect ROS,SOD,TNF-α,IL-1β,and IL-6 in cells.Flow cytometry was used to detect cell apoptosis.RT-qPCR were used to detect the mRNA levels of LncRNA KCNQ1OT1,miR-20a-5p,and PTEN mRNA in cells.Western blot experiments were used to detect PTEN and cell pro-liferation related proteins in cells.Dual-luciferase reporter gene assay was used to determine the targeting relationship between miR-20a-5p and LncRNA KCNQ1OT1,as well as between miR-20a-5p and PTEN.Results Compared with control group,OGD/R group exhibited a significantly reduced HT22 cell survival rate,as well as decreased SOD,miR-20a-5p,Ki67,and PCNA proteins(P＜0.05),while showing significantly increased ROS,TNF-α,IL-1β,IL-6,cell apoptosis rate,LncRNA KCNQ1OT1,PTEN mR-NA,and protein(P＜0.05).Compared with si-NC group or miR mimic-NC group,si-KCN or miR-20a-5p mimic group had a sig-nificantly increased cell survival rate,along with increased SOD,miR-20a-5p,Ki67,and PCNA proteins(P＜0.05),and signifi-cantly decreased ROS,TNF-α,IL-1β,IL-6,cell apoptosis rate,LncRNA KCNQ1OT1,PTEN mRNA,and protein(P＜0.05).Com-pared with si-KCN+miR inhibitor-NC group,si-KCN+miR-20a-5p inhibitor group had a significantly reduced cell survival rate,SOD,miR-20a-5p,Ki67,and PCNA(P＜0.05),and significantly increased ROS,TNF-α,IL-1β,IL-6,cell apoptosis rate,PTEN mRNA,and protein(P＜0.05).In the cells co-transfected with miR-20a-5p mimic and KCNQ1OT1-WT or PTEN-WT,the relative luciferase activity was significantly reduced(P＜0.05).Conclusion Downregulation of LncRNA KCNQ1OT1 may promote OGD/R-induced hippocampal neuronal cell proliferation,inhibit cellular oxidative stress and inflammatory damage by upregulating miR-20a-5p and reducing PTEN.关键词
长链非编码RNA KCNQ1OT1/微小RNA-20a-5p/磷酸酶和张力蛋白同源物轴/海马神经元Key words
long non coding RNA KCNQ1OT1/microRNA-20a-5p/phosphatase and tensin homologue deleted on chromo-some ten axis/hippocampal neurons分类
医药卫生引用本文复制引用
邱志雄,薛飞,王龙,顾伟杰,史轩丰..LncRNA KCNQ1OT1调节miR-20a-5p/PTEN轴对OGD/R诱导的海马神经元损伤的影响[J].四川医学,2026,47(3):279-286,8.基金项目
江阴市卫生健康委科研项目(编号:M202214) （编号:M202214）
