山东农业大学学报（自然科学版）2026，Vol.57Issue(2)：263-270,8.DOI:10.3969/j.issn.1000-2324.2026.02.007
黔北黑猪不同骨骼肌内脂肪沉积相关lncRNAs的鉴定
Identification of lncRNAs Related to Fat Deposition in Different Skeletal Muscle of Qianbei Black Pigs
摘要
Abstract
This study aims to explore the candidate lncRNAs related to intramuscular fat deposition in different types of skeletal muscle of Qianbei black pigs.Nine healthy female Qianbei black sows(180±11 days old and 60±7 kg in weight)with close kinship are randomly divided into 3 groups.Samples are collected from the longissimus dorsi and psoas major muscles.RNA-seq is used to construct lncRNA expression profiles,and differentially expressed lncRNAs are screened and validated by qRT-PCR.Bioinformatics methods are employed to predict the target genes of these differentially expressed lncRNAs,followed by GO and KEGG functional enrichment analyses to identify lncRNAs related to intramuscular fat deposition.Additionally,an lncRNA-mRNA regulatory network is constructed.A total of 4 232 lncRNAs are annotated in the two skeletal muscles of Qianbei black pigs,from which 45 differentially expressed lncRNAs are identified(23 downregulated and 22 upregulated).The qRT-PCR results indicate that the expression patterns are highly consistent with the RNA-seq data.GO and KEGG analysis reveal that the target genes of differentially expressed lncRNAs are mainly enriched in GO terms such as phospholipid,lipid and fatty acid transport,fatty acid breakdown,and negative regulation of estrogen receptor signaling pathways,as well as signaling pathways involving ABC transporters,endocytosis,bile secretion,metabolic pathways,and peroxisomes.The lncRNA-mRNA network indicates that TCONS_00123816 and TCONS_00014197 may regulate intramuscular fat deposition through the PPAR signaling pathway and the Wnt signaling pathway,respectively.TCONS_00123816 and TCONS_00014197 may regulate skeletal muscle fat deposition through their target genes in pig.These findings provide data support for in-depth analysis of the mechanisms of fat deposition in different types of skeletal muscle and hold significant implications for assisting in the breeding of high-quality meat varieties.关键词
黔北黑猪/lncRNAs/背最长肌/腰大肌/脂肪沉积Key words
Qianbei black pig/lncRNAs/longissimus dorsi muscle/psoas major muscle/fat deposition分类
农业科技引用本文复制引用
李琳,孟金柱,张虹亮..黔北黑猪不同骨骼肌内脂肪沉积相关lncRNAs的鉴定[J].山东农业大学学报（自然科学版）,2026,57(2):263-270,8.基金项目
贵州省科学技术厅高层次创新型人才培养项目(千层次)(2022-(2020)-044) （千层次）
