药学研究2026，Vol.45Issue(3)：275-280,6.DOI:10.13506/j.cnki.jpr.2026.03.005
牛蒡根总多酚提取工艺以及含量测定
Study on optimization of extraction technology and accurate content determination of total polyphenols from Arctium lappa L.root
摘要
Abstract
Objective To optimize the extraction process of total polyphenols from Arctium lappa L.roots,establish a purification and enrichment method,and determine the content of its characteristic marker components,so as to provide a basis for subsequent research.Methods With total polyphenol content(TPC)as the evaluation index,single-factor experiments were conducted to screen the optimal parameters of ethanol concentration,solid-to-liquid ratio,and extraction temperature.The crude extract was purified and enriched by sequential petroleum ether defatting,n-butanol extraction,and AB-8 type macroporous adsorption resin.Folin-Ciocalteu method was used to determine the TPC,and high-performance liquid chromatography(HPLC)was applied for the quantitative analysis of 7 marker phenolic acid components.Results The optimal extraction conditions were 70％ethanol,a solid-to-liquid ratio of 1∶15(g∶mL),and extraction at 60 ℃,under which the TPC of the crude extract reached 2.71％.After purification and enrichment,the TPC was significantly increased to 59.84％.HPLC analysis showed that the total content of 7 marker components(including neochlorogenic acid and chlorogenic acid)accounted for 28.05％of the purified extract.Conclusion The established extraction and enrichment processes are efficient and feasible,and the main component contents of the extract are clarified,which provides technical support for the development and utilization of Arctium lappa L.root polyphenols.关键词
牛蒡根/总多酚/工艺优化/大孔树脂富集/高效液相色谱法Key words
Arctium lappa L.root/Total polyphenols/Process optimization/Macroporous resin enrichment/High-performance liquid chromatography(HPLC)分类
医药卫生引用本文复制引用
张雅婷,郑彧,孙翌勋,曲扬..牛蒡根总多酚提取工艺以及含量测定[J].药学研究,2026,45(3):275-280,6.基金项目
辽宁省科技计划联合基金项目(No.2100224182) （No.2100224182）
