山东农业科学2026，Vol.58Issue(3)：16-24,9.DOI:10.14083/j.issn.1001-4942.2026.03.002
忍冬WD40基因家族鉴定及盐胁迫下的表达分析
Identification and Expression Analysis under Salt Stress of WD40 Gene Family in Lonicera japonica Thunb.
摘要
Abstract
The WD40 gene family plays significant roles in plant stress response and regulation of second-ary metabolite synthesis in various biological activities.In this study,272 WD40 genes of Lonicera japonica were identified and analyzed by bioinformatics methods,and their expression characteristics were also analyzed under NaCl stress.The results revealed that the 272 genes were distributed across all 9 pairs of L.japonica chromosomes,and encoded 60 to 3 457 amino acid residues.These WD40 proteins had molecular weights var-ying from 6 581.97 to 385 924.83 Da and aliphatic index from 48.23 to 105.76,and most of them exhibited a-cidic,unstable and hydrophilic characteristics.Evolutionary analysis results showed that the 272 genes could be divided into 7 clusters,and cluster Ⅰ to Ⅶ contained 25,37,43,34,37,44 and 52 members,respec-tively.The expression analysis of 10 L.japonica WD40 genes under different concentrations(100,200 and 300 mmol/L)of NaCl stress using real-time fluorescence quantitative PCR revealed that significant changes of expression level were observed in 8 of these genes,among which,6 showed an overall up-regulaing trend,1 showed down-regulation under lower NaCl concentration but up-regulation under higher NaCl concentration,and 1 showed decreasing first and then increasing,suggesting a potential association with salt tolerance of L.japonica.Comparative analysis of these changes indicated that these genes might have multiple functions at dif-ferent stages and states of salt stress,and some feedback regulation mechanisms might exist;they also might play multiple roles in the regulatory network.All these results could lay foundations for further research of the functions of WD40 gene family,and provide references for salt tolerant gene selection of L.japonica.关键词
忍冬/WD40基因/盐胁迫/生物信息学分析/表达分析Key words
Lonicera japonica/WD40 gene/Salt stress/Bioinformatics analysis/Expression analysis分类
农业科技引用本文复制引用
李启东,王飞,王彬,李雪洁,王刚,杨盛,黄璐瑶,刘振华,李佳..忍冬WD40基因家族鉴定及盐胁迫下的表达分析[J].山东农业科学,2026,58(3):16-24,9.基金项目
山东省重点研发计划(乡村振兴科技创新提振行动计划)项目(2022TZXD0036) （乡村振兴科技创新提振行动计划）
山东省博士后创新项目(SDCX-ZG-202400079) （SDCX-ZG-202400079）
