山东农业科学2026，Vol.58Issue(3)：25-30,6.DOI:10.14083/j.issn.1001-4942.2026.03.003
蒺藜苜蓿肌动蛋白家族基因的鉴定及表达模式分析
Identification and Expression Pattern of Actin Gene Family in Medicago truncatula
摘要
Abstract
Actin is a highly conserved protein widely distributed in eukaryotes.It forms microfilament cy-toskeletons through polymerization to support basic cell morphology and participates in regulating numerous growth and development processes.In the higher plants,actin-encoding genes typically exist as gene families,yet no such reports exist for the legume model plant Medicago truncatula.This study conducted a genome-wide identification of actin genes in M.truncatula,and analyzed their evolutionary relationships,sequence charac-teristics and expression patterns.The results indicated that the M.truncatula genome contained seven actin genes(MtACT1 to MtACT7),which distributed across five chromosomes.Proteins encoded by MtACTs all com-prised 377 amino acids,with sequence similarity exceeding 92％among different members.Based on evolu-tionary relationships,MtACTs could be grouped into three clusters,and the members within the same cluster exhibited similar gene structure.MtACTs displayed diverse expression patterns in different tissues and organs.Among them,MtACT2 and MtACT4 showed stable high expression in various tissues or organs,so they could serve as housekeeping genes for M.truncatula.These findings layed a foundation for further exploration of the biological functions of MtACTs.关键词
蒺藜苜蓿/肌动蛋白/基因家族/进化分析/表达模式Key words
Medicago truncatula/Actin/Gene family/Phylogenetic analysis/Expression pattern分类
农业科技引用本文复制引用
沙奇,侯君泽,周雯,孙永旺..蒺藜苜蓿肌动蛋白家族基因的鉴定及表达模式分析[J].山东农业科学,2026,58(3):25-30,6.基金项目
国家自然科学基金项目(32101788) （32101788）
聊城大学博士科研启动基金项目(318052021) （318052021）
聊城大学校企合作资金资助项目(K25LD101) （K25LD101）
