山东农业科学2026，Vol.58Issue(3)：31-36,6.DOI:10.14083/j.issn.1001-4942.2026.03.004
高浓度Zebularine诱发小偃麦染色体变异研究
Chromosomal Variations in Trititrigia Induced by High Concentration of Zebularine
摘要
Abstract
Chemical mutagenesis is one of the important approaches to induce plant trait variation.Cur-rently,the most commonly used chemical mutagen is ethyl methyl sulfonate(EMS).However,there are few reports on the use of cytosine analog Zebularine for induction and selection of superior germplasm resources in cereal crops.In this study,high concentration of Zebularine was used as the mutagen to treat germinated seeds of three Trititrigia lines(XY78829,XY7430,and XY96253).The results showed that abundant chromosomal structural variations were observed in the metaphase cells of Trititrigia root tips compared with the negative control(ddH2O),mainly including chromosomal translocations(429,59.0％)and deletions or fragments(298,41.0％).The frequency of intergenomic translocations(380,52.3％)was significantly higher than that of intragenomic translocations(49,6.7％).Within intergenomic translocations,the most translocations oc-curred between homeologous group A and B(111,15.3％).These conclusions could provide valuable infor-mation for further transfer and utilization of beneficial genes from Thinopyrum.关键词
小偃麦/Zebularine/化学诱变/染色体变异Key words
Trititrigia/Zebularine/Chemical mutagenesis/Chromosomal variation分类
农业科技引用本文复制引用
王青,丁智超,孙佳凯,鲍印广,马旭辉,王玉海..高浓度Zebularine诱发小偃麦染色体变异研究[J].山东农业科学,2026,58(3):31-36,6.基金项目
山东省重点研发计划(重大科技创新工程)项目(2021LZGC009) （重大科技创新工程）
大学生创新创业训练计划项目(S202510904030) （S202510904030）
