山东医药2026，Vol.66Issue(3)：6-9,14,5.DOI:10.3969/j.issn.1002-266X.2026.03.002
双原发恶性肿瘤的胃癌患者肿瘤细胞差异表达基因分析
Analysis of differentially expressed genes in gastric cancer patients with double primary malignant tumors
摘要
Abstract
Objective To screen and analyze the differentially expressed genes(DEGs)in tumor cells of gastric cancer patients with double primary malignant tumors,in order to explore the possible molecular mechanisms of double pri-mary malignant tumors in patients with gastric cancer.Methods Eighty gastric cancer patients admitted from June 2023 to December 2024 were selected,including 40 patients with single primary malignant tumor and 40 patients with double prima-ry malignant tumors(with primary malignant tumors at other unrelated sites).Single-cell suspensions were prepared from tumor tissues collected during surgery,and single-cell sequencing libraries were constructed for high-throughput sequencing.After identifying tumor cells,the gene expression profiles of tumor cells between the two groups were compared,and DEGs were screened using the MAST algorithm.Gene Ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)signaling pathway enrichment analysis were performed on the screened DEGs.Results A total of 1,865 DEGs were identified between the two groups.Among them,1,023 genes were up-regulated(e.g.,EGFR)and 842 genes were down-regulated(e.g.,PTEN)in patients with double primary malignant tumors.GO enrichment analy-sis showed that DEGs were significantly enriched in biological processes such as cell proliferation,migration,and regulation of immune response;in biological functions including regulation of protein kinase activity and transcription factor binding;and in cellular components such as extracellular matrix components and cell membrane receptor complexes.KEGG enrich-ment analysis revealed that DEGs were significantly enriched in the PI3K-AKT signaling pathway,MAPK signaling path-way,Wnt signaling pathway,and PD-1/PD-L1 signaling pathway.Multiple related genes in these four pathways were up-regulated in patients with double primary malignant tumors(e.g.,PIK3CA,AKT1,MAPK1,MAPK3,CTNNB1,PD-L1).Conclusions Multiple DEGs exist in tumor cells of gastric cancer patients with double primary malignant tumors.These DEGs are mainly enriched in key biological processes such as regulation of cell proliferation and migration,regulation of immune response,and cellular signal transduction.Several cellular signaling pathways(e.g.,PI3K-AKT,MAPK,Wnt signaling pathways)are abnormally activated in double primary malignant tumors.关键词
胃癌/双原发恶性肿瘤/单细胞测序Key words
gastric carcinoma/double primary malignant neoplasms/single-cell RNA sequencing分类
医药卫生引用本文复制引用
周杨,张璐璐,朱元增..双原发恶性肿瘤的胃癌患者肿瘤细胞差异表达基因分析[J].山东医药,2026,66(3):6-9,14,5.基金项目
河南省医学科技攻关项目(LHGJ20230077). （LHGJ20230077）
