饲料工业2026，Vol.47Issue(8)：133-141,9.DOI:10.13302/j.cnki.fi.2026.08.018
八珍散总皂苷生物提取的工艺优化及抗氧化活性研究
Optimization of Biological Extraction Process and Study on Antioxidant Activity of Total Saponins from Bazhen San
摘要
Abstract
This experiment aimed to improve the extraction efficiency and antioxidant activity of total sapo-nins from Bazhen San using solid-state fermentation with Lactobacillus plantarum and Bifidobacterium ani-malis.The effects of fermentation time,temperature,moisture content,and inoculum size on the extraction yield of total saponins were systematically studied through single-factor tests and an orthogonal array design.Antioxidant activity was evaluated by mea-suring 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging rate,2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)(ABTS+)radical scavenging rate,and reducing power.Single-factor test results indicated that the maximum saponin extraction was achieved under the follow-ing conditions:a time of 48 h,a temperature of 34℃,a moisture content of 50%,and an inoculum size of 1×108 CFU/g.The increase was significant(P<0.05).Orthogonal test results showed the optimal fermenta-tion parameters were:a time of 48 h,a temperature of 31℃,a moisture content of 50%,and an inoculum size of 1×109 CFU/g.Under these optimal conditions,the total saponin extraction from the fermented prod-uct was significantly increased compared to the unfermented material(P<0.05).The primary and secondary factors affecting saponin yield during fermentation are,in descending order of importance:moisture con-tent>inoculation amount>fermentation temperature>fermentation time.Furthermore,the fermented prod-uct demonstrated superior antioxidant activity,showing higher DPPH radical scavenging rate,ABTS⁺ radical scavenging rate,and reducing power.The results demonstrate that solid-state fermentation can effectively enhance the total saponin content and antioxidant activity of Bazhen San.关键词
八珍散/固态发酵/工艺优化/总皂苷/抗氧化活性Key words
Bazhen San/solid-state fermentation/process optimization/total saponins/antioxidant activity分类
农业科技引用本文复制引用
赵增辉,要纬玉,王霄,包永占,王鹏,房思远,史万玉,李建喜..八珍散总皂苷生物提取的工艺优化及抗氧化活性研究[J].饲料工业,2026,47(8):133-141,9.基金项目
"十四五"国家重点研发计划项目[2022YFD1801104] （）
国家现代农业产业技术体系项目[CARS-36] （）
河北省现代农业产业技术体系创新团队建设专项[HBCT2024110202] （）
