世界科学技术-中医药现代化2026，Vol.28Issue(4)：1063-1073,11.DOI:10.11842/wst.20251211008
葛根素/液态金属水凝胶的制备及其介导三阴性乳腺癌光热治疗研究
Preparation of Puerarin/Liquid Metal Hydrogel and Its Application in Photothermal Therapy of Triple-Negative Breast Cancer
摘要
Abstract
Objective To construct a novel puerarin/liquid metal(Pue/LM)hydrogel for the photothermal therapy of triple-negative breast cancer(TNBC).Methods A stable Pue/LM hydrogel was fabricated by optimizing the ratio of Pue to LM.Its structural characteristics and properties were systematically characterized using scanning electron microscopy(SEM),energy-dispersive spectroscopy(EDS),Fourier transform infrared spectroscopy(FTIR),X-ray diffraction(XRD),and ultraviolet-visible spectroscopy(UV-vis).The photothermal conversion efficiency was calculated based on temperature changes under 1064 nm near-infrared(NIR)light irradiation,and photothermal response stability was verified via cyclic heating-cooling experiments.In vivo photothermal efficacy was evaluated using animal models.Additionally,live/dead cell staining,flow cytometry,and mitochondrial membrane potential detection were combined to investigate the photothermal killing effect and underlying mechanism of Pue/LM on 4T1 cells under NIR light irradiation.Results The Pue/LM hydrogel was successfully constructed with optimized proportions,achieving efficient loading of LM.Under 1064 nm NIR light irradiation,the hydrogel exhibited significant photothermal conversion capacity and excellent cyclic stability.In vivo experiments confirmed its photothermal response at the tumor site.Cellular studies demonstrated that,under NIR light irradiation,the Pue/LM hydrogel remarkably increased the late apoptosis and necrosis rates of 4T1 tumor cells by reducing mitochondrial membrane potential and disrupting mitochondrial function.Conclusions This study successfully developed a novel Pue/LM hydrogel,which shows promising therapeutic potential for TNBC under photothermal conditions,providing a new strategy for the local treatment of this disease.关键词
三阴性乳腺癌/葛根素/液态金属/水凝胶/光热性能Key words
TNBC/Puerarin/Liquid metal/Hydrogel/Photothermal properties分类
医药卫生引用本文复制引用
侯义冲,王涛,张超,邓万林,邱崇,王继刚,夏慧..葛根素/液态金属水凝胶的制备及其介导三阴性乳腺癌光热治疗研究[J].世界科学技术-中医药现代化,2026,28(4):1063-1073,11.基金项目
山东省科学技术厅山东省自然科学基金(ZR2023MF021):脑卒中血流-电导率快速检测的磁声电成像新方法,负责人:夏慧. （ZR2023MF021）
