兽类学报2026，Vol.46Issue(2)：153-170,18.DOI:10.16829/j.slxb.151026
塔里木马鹿PRDX1基因表达载体的构建及其生物信息学分析
Construction of the expression vector for the PRDX1 gene in Tarim red deer and its bioinformatic analysis
摘要
Abstract
To investigate the structural and functional characteristics of Peroxiredoxin 1(PRDX1),which may play an antioxidant role in the adaptation of the Tarim red deer(Cervus elaphus yarkandensis)to arid environments,this study amplified the coding sequence(CDS)of the PRDX1 gene(CeyPRDX1)using reverse transcription PCR(RT-PCR).The CDS sequence was successfully cloned into the pcDNA3.1(+)vector to construct an expression vector and named pcD-NA3.1(+)-CeyPRDX1.The pLJM1-EGFP Lentivirus vector with EGFP fluorescent label was used to construct overex-pression vector of CeyPRDX1(pLJM1-CeyPRDX1-EGFP),and its subcellular localization was observed in the cyto-plasm and nucleus of human HaCaT cells.The results of homology comparison and phylogenetic tree analysis indicated that CeyPRDX 1 has the highest homology and the closest phylogenetic relationship with Eastern European red deer(Cer-vus elaphus)and red deer(Cervus canadensis).Bioinformatic analysis revealed that CeyPRDX 1 is a conserved,hydro-philic protein without N-glycosylation or transmembrane regions,and interacts with various antioxidant proteins.This study provides a foundation for future functional research on the CeyPRDX1 gene.关键词
塔里木马鹿/PRDX1基因/载体构建/生物信息学分析Key words
Tarim red deer/PRDX1/Vector construction/Bioinformatic analysis分类
生物科学引用本文复制引用
布威海丽且姆·阿巴拜科日,艾比布拉·阿布都拉,布海力切木·依明艾力,赵华斌..塔里木马鹿PRDX1基因表达载体的构建及其生物信息学分析[J].兽类学报,2026,46(2):153-170,18.基金项目
新疆和田学院校级科研培育项目(2025ZR008) （2025ZR008）
新疆维吾尔自治区自然科学基金(2022D01B40) （2022D01B40）
