兽类学报2026，Vol.46Issue(2)：184-198,15.DOI:10.16829/j.slxb.151022
狗獾全基因组微卫星分布规律及特征分析
Analysis of the distribution patterns and characteristics of microsatellites in Meles meles genome
摘要
Abstract
The Eurasian badger(Meles meles)belongs to the order Carnivora and the genus Meles.It lives in social groups in underground environments and exhibits distinct territorial behaviors.The Eurasian badger is primarily noctur-nal,with a broad dietary preference,and it is also an important wildlife host for bovine tuberculosis.This study systemat-ically investigates the distribution characteristics of microsatellites in the Eurasian badger genome using bioinformatics methods and annotates and functionally analyzes genes containing microsatellites in coding regions.The results show that the Eurasian badger genome is 2.74 Gb in size and contains 975 935 microsatellites,with a relative abundance of 356.35 loci/Mb.The distribution pattern of microsatellites across the whole genome is as follows:mononucleotide＞di-nucleotide＞tetranucleotide＞trinucleotide＞pentanucleotide＞hexanucleotide repeats.Among these,dinucleotide re-peats have the highest GC content,while mononucleotide repeats have the lowest.Microsatellites with A/T-rich sequenc-es dominate in the genome.The abundance of microsatellites varies in different regions of the genome,with the highest number found in intergenic regions(595 769),the highest relative abundance in introns(385.22 loci/Mb),and the lowest in coding regions(1 724 loci and 48.40 loci/Mb).The distribution characteristics of microsatellites in intergenic regions are similar to those in the whole genome,while the exons show a higher frequency of trinucleotide repeats,with a strong preference for trinucleotide and hexanucleotide repeat types in the coding regions.Regarding the positional features of microsatellite abundance,the highest abundance is found in the intron regions,followed by the 500 bp upstream and downstream regions of genes,with the lowest in the exonic regions.There are 1 724 CDSs containing microsatellites in the Eurasian badger genome,with 1 325 genes annotated in the Gene Ontology(GO)database,which are assigned to 11 971 GO terms and categorized into 39 major functional groups.The most significantly enriched Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway is the signaling pathway,containing 207 genes.This study provides fundamen-tal data for the subsequent development of high-quality microsatellite markers for the Eurasian badger and offers a refer-ence for understanding the functional and distribution characteristics of microsatellites in the whole genome of carnivo-rous species.关键词
狗獾/微卫星/分布规律/GO分析/KEGG富集Key words
Meles meles/Microsatellite/Distribution patterns/GO analysis/KEGG enrichment分类
生物科学引用本文复制引用
郗鸿原,刘文静,窦海龙..狗獾全基因组微卫星分布规律及特征分析[J].兽类学报,2026,46(2):184-198,15.基金项目
国家自然科学基金青年基金(31800452) （31800452）
质兰公益基金(2022070461B) （2022070461B）
