饲料研究2026，Vol.49Issue(6)：81-88,8.DOI:10.13557/j.cnki.issn1002-2813.2026.06.014
基于LC-MS结合网络药理学、分子对接及体内验证探究"桂南"翅荚决明对鸡抗炎作用机制
Elucidating anti-inflammatory mechanism of Senna alata'Guinan'in chickens based on LC-MS combined with network pharmacology,molecular docking,and in vivo validation
摘要
Abstract
This study aimed to investigate the regulatory mechanism of the leguminous forage Senna alata'Guinan'on the inflammatory responses in chickens.The main active ingredients were identified using liquid chromatography-mass spectrometry(LC-MS)technology,and potential targets and signaling pathways were predicted using network pharmacology.Molecular docking and in vivo experiment were used for validation.The results showed that LC-MS analysis identified nine major active components,including galangin,6-hydroxyluteolin,morin,fisetin,and kaempferol.Database screening predicted 179 potential targets,which were intersected with 2 466 chicken inflammation-related targets,yielding 101 common targets.Protein-protein interaction(PPI)network further screened 19 core targets,including protein kinase B serine/threonine kinase 1(AKT1),epidermal growth factor receptor(EGFR),and B-cell lymphoma/leukemia-2 gene(BCL2).Gene Ontology(GO)functional enrichment analysis indicated that these targets were primarily involved in biological processes such as protein phosphorylation and apoptosis.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis revealed significant enrichment in anti-inflammatory-related signaling pathways,including the Toll-like receptor signaling pathway and apoptosis.Molecular docking results demonstrated strong binding affinity between the aforementioned active components and the core targets.In vivo experiment indicated that feeding a diet containing 20%Senna alata'Guinan'reduced serum levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β)in chickens.The study shows that clarifing the core active components,targets,and mechanisms by which Senna alata'Guinan'regulates inflammation in chickens,providing a reference for its development and application as an anti-inflammatory forage resource in chicken farming.关键词
"桂南"翅荚决明/网络药理学/分子对接/LC-MS/鸡/炎症Key words
Senna alata'Guinan'/network pharmacology/molecular docking/LC-MS/chickens/inflammation分类
农业科技引用本文复制引用
梁琪妹,吴冯莹,赖大伟,李怡丽,卢健琴,李柱秋,梁杰豪,姚娜..基于LC-MS结合网络药理学、分子对接及体内验证探究"桂南"翅荚决明对鸡抗炎作用机制[J].饲料研究,2026,49(6):81-88,8.基金项目
中央引导地方科技发展专项(项目编号:桂科ZY21195051) （项目编号:桂科ZY21195051）
广西农业职业技术大学校级产业创新团队专项(项目编号:XTKJ2502-2、XTKJ2502-4) （项目编号:XTKJ2502-2、XTKJ2502-4）
