饲料研究2026，Vol.49Issue(6)：102-108,7.DOI:10.13557/j.cnki.issn1002-2813.2026.06.017
白蔹多酚超声波辅助酶法提取工艺优化及抗氧化、抑菌活性研究
Optimization of ultrasonic-assisted enzymatic extraction of Ampelopsis radix polyphenols and study on its antioxidant and antioxidant activities
摘要
Abstract
The experiment aimed to optimize the ultrasonic-assisted enzymatic extraction process of Ampelopsis radix polyphenols(Arp)and study on its antioxidant and antibacterial activities.By conducting a single factor experiment to investigat the effects of liquid to material ratio,enzymatic hydrolysis temperature,ultrasound time,and enzyme addition on the extraction rate of Arp,and the extraction process was optimized by central composite design response surface method with Design Expert software.The scavenging capacities of the extraction on 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)radicals and 2,2-benzyl-bis-(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS)radicals,and the antibacterial activity against Staphylococcus aureus and Escherichia coli were determined.The results showed that the optimum technological conditions were as follows:Liquid to material ratio of 33.00 mL/g,enzymolysis temperature of 45.00℃,ultrasonic time of 54.00 min and enzyme addition amount of 3.50%.Under these conditions,the extraction rate of Arp was 35.57%[relative standard deviation(RSD)=1.68%],with a deviation of 1.35%from the predicted value.The scavenging rates of DPPH radicals and ABTS radicals were 89.54%and 72.98%respectively,and the half maximal inhibitory concentration(IC50)value were 0.68 g/L and 0.86 g/L respectively.Arp exhibited good inhibitory effects against Staphylococcus aureus and Escherichia coli,with the minimum inhibitory concentration(MIC)of 3.125 000 g/L and 1.562 500 g/L,respectively.The study shows that ultrasonic-assisted enzymatic extraction process is simple,controllable,stable and feasible.The extracted Arp has strong antioxidant and antibacterial activities,and can be used as a feed additive in the livestock and poultry breeding industry.关键词
白蔹/多酚/中心复合设计/响应面/抗氧化/抑菌活性Key words
Ampelopsis radix/polyphenols/central composite design/response surface/antioxidant/antimicrobial activity分类
农业科技引用本文复制引用
游国叶,史琼,汪学猛,杜晶,王海燕,张存良..白蔹多酚超声波辅助酶法提取工艺优化及抗氧化、抑菌活性研究[J].饲料研究,2026,49(6):102-108,7.基金项目
河南省科技厅科技攻关项目(项目编号:242102310575) （项目编号:242102310575）
河南省重点研发专项(项目编号:241111311400) （项目编号:241111311400）
信阳职业技术学院重点项目(项目编号:LXZD251103) （项目编号:LXZD251103）
