生物技术进展2026，Vol.16Issue(2)：439-451,13.DOI:10.19586/j.2095-2341.2025.0155
基于网络药理学和孟德尔随机化探究硬尖神香草治疗哮喘的作用机制
Based on Network Pharmacology and Mendelian Randomization to Investigate the Mechanism of Hyssopus cuspidatus Boriss.in the Treatment of Asthma
摘要
Abstract
This study aims to systematically investigate the active components,target genes,and molecular mechanisms of Hysso-pus cuspidatus Boriss.in the treatment of asthma,thereby providing a scientific basis for its clinical application.A comprehensive metabolomic analysis of Hyssopus cuspidatus Boriss.was conducted using dual-platform UPLC-MS/MS and GC-MS to identify its active components.Potential therapeutic targets were screened using databases such as TCMSP,PubChem,and SwissTargetPre-diction.Asthma-related targets were retrieved from GeneCards,OMIM,and TTD databases.A protein-protein interaction(PPI)network was constructed,followed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses.Molecular docking was performed to validate the binding affinity between active components and core targets.Finally,a two-sample Mendelian randomization(MR)analysis was employed to verify the causal relationship between core targets and asth-ma.The results showed that a total of 79 active components and 410 potential targets were identified in Hyssopus cuspidatus Boriss.Intersection with asthma-related targets yielded 76 common targets.PPI network analysis revealed AKT1,STAT3,EG-FR,PTGS2,and SRC as core targets.GO and KEGG enrichment analyses indicated significant enrichment in processes such as the PI3K/AKT signaling pathway,inflammatory response,and apoptosis.Molecular docking results demonstrated strong binding affinities between flavonoid components(e.g.,luteolin and isorhamnetin)and core targets.Mendelian randomization analysis fur-ther confirmed a significant causal relationship between AKT1 and asthma.Hyssopus cuspidatus Boriss.might exert therapeutic ef-fects on asthma through multiple flavonoid active components targeting core genes such as AKT1,regulating signaling pathways like PI3K/AKT,thereby suppressing airway inflammation and alleviating airway hyperresponsiveness.AKT1 is identified as a key target with potential clinical translational value.关键词
硬尖神香草/哮喘/孟德尔随机化/网络药理学Key words
Hyssopus cuspidatus Boriss./asthma/Mendelian randomization/network pharmacology分类
农业科技引用本文复制引用
李名驰,史增芳,张国帅,颜齐,王嘉乐,张新可,黄林芳..基于网络药理学和孟德尔随机化探究硬尖神香草治疗哮喘的作用机制[J].生物技术进展,2026,16(2):439-451,13.基金项目
内蒙古自治区科技计划(2025YFHH0227) （2025YFHH0227）
中国医学科学院医学科学创新基金项目(2022-2M-1-017) （2022-2M-1-017）
国家自然科学基金项目(82274045 （）
82211540726 （）
82073960 （）
U1812403-1) （）
北京市自然科学基金项目(7242244) （7242244）
新疆维吾尔自治区重点研发专项(2023B02022-1). （2023B02022-1）
