生物技术通报2026，Vol.42Issue(3)：263-274,12.DOI:10.13560/j.cnki.biotech.bull.1985.2026-0160
番茄VPE基因家族鉴定和抗逆功能分析
Identification of VPE Gene Family and Their Functional Analysis under Abiotic Stress in Tomato
摘要
Abstract
[Objective]To systematically identify the members of vacuolar processing enzyme(VPE)gene family in tomato,and elucidate its evolutionary relationships,expression patterns,and potential functions in response to abiotic stress.[Method]Members of the SlVPE family were identified using bioinformatics methods.Systematic analyses were performed on their physicochemical properties,phylogenetic relationships,gene structures,conserved domains,chromosomal distribution,cis-acting elements,and interspecific collinearity.Public transcriptome data and RT-qPCR were integrated to analyze the tissue-specific expression patterns of the SlVPE family and their expression responses to drought,high/low temperature,salt,alkali,and combined salt-alkali stress.[Result]A total of 13 SlVPE genes containing both Peptidase_C13 and legumain_C domains were identified in tomato and classified into three subfamilies.With the exception of SlVPE4 and SlVPE5,all members were clustered in a gene cluster on chromosome 8.Expression analysis revealed significant tissue and spatiotemporal specificity within the SlVPE family:SlVPE3 and SlVPE5 were constitutively highly expressed,while SlVPE2 and SlVPE7 were fruit-specific highly expressed genes.Under abiotic stress,the family's response was predominantly characterized by transcriptional suppression.Notably,SlVPE3 emerged as a core responsive gene to drought,salt,and combined salt-alkali stress,SlVPE7 was specifically induced by alkali stress,and SlVPE8 primarily responded to high and low-temperature stress.Based on sequence variations between wild tomato(Solanum pimpinellifolium)and cultivated tomato(S.lycopersicum),four polymorphic molecular markers were developed in the promoter regions or downstream regions of SlVPE gene.[Conclusion]The tomato SlVPE gene family consists of 13 members,11 of which are clustered on chromosome 8.SlVPE3 and SlVPE5 are constitutively highly expressed genes,while SlVPE2 and SlVPE7 exhibit specific high expression during early fruit development.In contrast,abiotic stresses primarily induce the expressions of SlVPE3,SlVPE7,and SlVPE8.关键词
番茄/液泡加工酶/顺式作用元件/非生物胁迫/基因簇/启动子/分子标记/醋栗番茄Key words
tomato/vacuolar processing enzyme/cis-acting element/abiotic stress/gene cluster/promoter/molecular marker/Solanum pimpinellifolium引用本文复制引用
李迎辉,王杨博涵,周浩博,卢心如,张珂欣,于洋,李传友,孙传龙..番茄VPE基因家族鉴定和抗逆功能分析[J].生物技术通报,2026,42(3):263-274,12.基金项目
山东省自然科学基金项目(ZR2024QC172,ZR2024QC154),国家自然科学基金项目(32302567),山东省泰山学者(TSQN202312148) （ZR2024QC172,ZR2024QC154）
