生物技术通报2026，Vol.42Issue(3)：312-323,12.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0816
'红阳'猕猴桃BGAL基因家族的全基因组鉴定与表达分析
Genome-wide Identification and Expression Analysis of BGAL Gene Family in Actinidia chinensis var.Hongyang
摘要
Abstract
[Objective]This study is aimed to conduct a genome-wide identification and expression pattern analysis of the β-galactosidase gene family(AcBGALs)in'Hongyang'kiwifruit(Actinidia chinensis var.Hongyang),providing a theoretical basis for further elucidating the functions of this gene family.[Method]The AcBGAL genes were identified based on the whole-genome sequence of'Hongyang'kiwifruit.Their physicochemical properties,chromosomal localizations,gene structures,phylogenetic relationships,and gene collinearity were analyzed using bioinformatics methods.Transcriptomic data and quantitative real-time PCR(RT-qPCR)were combined to investigate the expression patterns of these genes in different tissues,various fruit developmental stages,postharvest softening,and hormone response processes.[Result]A total of 21 BGAL family members were identified in the'Hongyang'kiwifruit genome,which belonged to six subfamilies and unevenly distributed across 14 chromosomes.Cis-acting element analysis revealed that the promoter regions of this gene family were enriched with elements related to growth/development and hormone response.Expression analysis showed that five of the six candidate genes(excluding AcBGAL3)were highly expressed in the fruit.Throughout fruit development,the expressions of AcBGAL2 and AcBGAL8 were significantly upregulated,whereas that of AcBGAL13 was downregulated,a trend consistent with transcriptomic analysis.During ripening and softening,five AcBGAL genes(excluding AcBGAL12)showed an upward trend in expression.Additionally,ethylene(ET)and abscisic acid(ABA)treatments downregulated the expressions of AcBGAL2,AcBGAL8 and AcBGAL12 in fruit.Treatment with forchlorfenuron(CPPU)and gibberellin(GA ₃)downregulated the expressions of AcBGAL2 and AcBGAL12 but upregulated the expressions of AcBGAL3,AcBGAL10 and AcBGAL13 in the early stages of treatment.[Conclusion]A total of 21 AcBGAL genes were identified in the'Hongyang'kiwifruit genome.Among them,six candidate genes showed expression patterns closely associated with fruit development and post-ripening softening,and were regulated by exogenous hormones(ET,ABA,CPPU,and GA₃).These findings provide a foundation for further functional characterization of AcBGAL genes.关键词
'红阳'猕猴桃/β-半乳糖苷酶(BGAL)/全基因组鉴定/生物信息学/表达分析/果实生长发育/果实后熟软化/激素处理Key words
Actinidia chinensis var.Hongyang/β-galactosidase(BGAL)/genome-wide identification/bioinformatics/expression analysis/fruit growth and development/fruit ripening and softening/hormone treatment引用本文复制引用
刘林娅,刘欢艳,梁鑫钰,宋姝熠,何斌,王绪英,黄亚成..'红阳'猕猴桃BGAL基因家族的全基因组鉴定与表达分析[J].生物技术通报,2026,42(3):312-323,12.基金项目
六盘水师范学院科研培育项目(LPSSY2023KJZDPY07),国家自然科学基金项目(32360740),贵州省教育厅自然科学研究项目(黔教技[2022]054号),六盘水市科技计划项目(52020-2023-0-2-15),大学生创新训练计划项目(S2024109771744),六盘水师范学院2024年度联合培养研究生专项科研项目(LPSSYLPY202422) （LPSSY2023KJZDPY07）
