生物技术通报2026，Vol.42Issue(4)：101-113,13.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0928
大豆CAD基因家族的鉴定及表达分析
Identification and Expression Analysis of CAD Gene Family in Soybean(Glycine max(L.)Merr.)
摘要
Abstract
[Objective]The characteristics and expression analysis under different stresses of the Cinnamyl Alcohol Dehydrogenase(CAD)gene family(GmCAD)in soybean were studied,providing a theoretical basis for further research on the biological function of the GmCADs gene family.[Method]Based on the soybean genome data,the CAD gene family members were screened and identified using bioinformatics methods.The characteristics of the encoded proteins,phylogenetic relationships,gene structure and conserved motifs were analyzed.[Result]A total of 18 GmCADs(GmCAD1-GmCAD18)were identified in the whole soybean genome,which were unevenly distributed on 13 chromosomes,encoding 219 to 364 amino acids.Phylogenetic analysis revealed that the GmCADs gene family can be divided into four subfamilies,and the genes within the same subfamily share similar gene structures and conserved motifs.The collinearity analysis indicates that fragment duplication was the main form of expansion in the GmCADs gene family.Selection pressure analysis showed that the GmCADs gene was under purifying selection.The promoters of the GmCADs gene family containing abundant cis-acting elements were mainly associated with the light response,hormone response,stress response and growth development processes.The protein interaction network prediction results showed a complex protein interaction network,which was formed by all GmCADs genes through five indirect proteins,and GO functional analysis significantly enriched in terms such as CAD activity and lignin metabolism process.The GmCAD members demonstratecertain selectivity in expression under different tissues and stress conditions(such as drought,salt,cold,shade,and high temperature stress).RT-qPCR validation indicates the expressions of nine GmCAD significantly changed at different time points under salt and drought treatments,mainly showing upregulation.[Conclusion]The distribution,structure and function of the 18 soybean CAD members are diverse,and the GmCADs gene may respond to abiotic stress during the growth and development of soybean plants.关键词
大豆/肉桂醇脱氢酶/基因家族/蛋白互作网络/表达分析Key words
soybean/CAD/gene family/protein interaction network/expression analysis引用本文复制引用
苏燕竹,李达,张爱爱,刘永光,张秀荣,薛其勤..大豆CAD基因家族的鉴定及表达分析[J].生物技术通报,2026,42(4):101-113,13.基金项目
山东省自然科学基金项目(ZR2024QC082),潍坊科技发展计划(2023GX052) （ZR2024QC082）
