生物技术通报2026，Vol.42Issue(4)：141-152,12.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0657
基于RNA-seq筛选番茄防御南方根结线虫病的差异基因与RPP13的表达特征分析
Screening of Differentially Expressed Genes in Tomato Defense against Meloidogyne incognita Based on RNA-seq and Expression Characteristics Analysis of RPP13 Gene
摘要
Abstract
[Objective]By conducting RNA-seq and weighted gene co-expression network analysis on resistant and susceptible tomato materials inoculated with root-knot nematodes(RKNs)(Meloidogyne incognita),we aim to focus on the signaling pathways and genes related to disease resistance,verify the root expression patterns of target genes,and provide theoretical basis for subsequent molecular function research on these genes.[Method]The resistant lines'18060'and susceptible lines'Moneymaker'were used for inoculation,and root samples were collected for transcriptome sequencing and WGCNA at the time points of no inoculation,2 d and 4 d after inoculation The main pathways and regulatory genes that might be involved in disease resistance were screened,and the determination of the tissue-specific expression and root expression of these target genes were completed.[Result]As the inoculation time prolonged,the number of differentially expressed genes(DEGs)gradually increased.In the comparison between the resistant and susceptible groups at 2 dpi,1 900 DEGs were screened out,and at 4 dpi the number of DEGs increased to 3 012.KEGG analysis showed that the"plant-pathogen interaction"was significantly enriched in both the within-group and between-group comparisons.In the between-group comparison,the DEGs enriched in this pathway mainly encoded receptor-like proteins/kinase,calcium signaling-related proteins,heat shock proteins,and transcription factors,etc.Among which 5 R genes encoding RPP13 protein were also screened out.Focusing on the DEGs during the inoculation period,the significant module MEbrown was screened out via the WGCNA,and the top 10 nodes based on the in degree and out degree were selected as hub genes based on the in degree and out degree.Two DEGs encoding RPP13 were found among them.The tissue-specific expression and root expression pattern identification further indicated that Solyc07g039410.3 might play a key role in tomato defense against the invasion of M.incognita.[Conclusion]Six RPP13 genes potentially involved in the process of resisting M.incognita are screened out.Among them,Solyc07g039420.1 and Solyc07g039410.3 demonstrate specific expression characteristics in the roots.At 4 dpi,the hub gene Solyc07g039410.3 is significantly induced and its expression increases by 6.23 times,indicating this gene plays a critical role in the defense of tomato against M.incognita.关键词
番茄/根结线虫/抗病/RPP13基因/RNA-seq/WGCNA/根部表达模式Key words
tomato/root-knot nematode/disease resistance/RPP13 gene/RNA-seq/WGCNA/root expression pattern引用本文复制引用
冯成蒿,党雨乐,王志泽,聂蔚丹,杨中敏,杜崇..基于RNA-seq筛选番茄防御南方根结线虫病的差异基因与RPP13的表达特征分析[J].生物技术通报,2026,42(4):141-152,12.基金项目
国家自然科学基金项目(32302652),校级研究生创新创业项目(XJAUGRI2025071) （32302652）
