生物技术通报2026，Vol.42Issue(4)：202-215,14.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0846
基于表型性状与SSR标记的香橼种质资源遗传多样性分析
Genetic Diversity Analysis of Citri Fructus Germplasm Resources Based on Phenotypic Traits and SSR Markers
摘要
Abstract
[Objective]Citri fructus germplasm resources in China were investigated to analyze phenotypic variation of fruit traits,genetic diversity,population structure and genetic differentiation characteristics,providing a scientific basis for germplasm conservation and superior cultivar breeding.[Method]The total of 40 batches of fruits and 90 leaves samples of plants from 16 provinces(37 cities)of China were used as the test materials,and then the genetic diversity analysis was conducted using 15 phenotypic traits and screened 10 pairs of SSR markers.[Result]The Shannon-Wiener index H and Simpson's diversity index D of 7 quality traits of citri fructus from different regions varied from 0.526 to 1.365 and 0.349 to 0.711,respectively.Among the 8 quantitative traits coefficient of variation ranged from 21%(number of segments)to 99%(single-fruit weight).According to phenotypic traits,the tested materials could be divided into three groups:Ⅰ(Xiangyuan and Zhique),Ⅱ(Xiangpao)and Ⅲ(Juyuan).The 10 pairs of primers with good polymorphism and stable bands were screened for PCR of SSR primers.The results showed that tthe number of alleles(Na),the number of effective alleles(Ne),Shannon's diversity index(I),observed heterozygosity(Ho),and expected heterozygosity(He)at the locus level were 1.832,1.354,0.331,0.824,and 0.210,respectively.By clustering,population structure and PCoA analysis,the 90 germplasm were divided into four groups.Xiangyuan and Zhique were further separated.The genetic diversity of the four groups was different with the descending order of Zhique(Ho=0.682,He=0.199),Juyuan(Ho=0.659,He=0.175),Xiangpao(Ho=0.436,He=0.133)and Xiangyuan(Ho=0.391,He=0.127).The Mantel test showed that the genetic distance of the citri fructus and geographical distance of the provenances were significantly correlated(r=0.558,P=0.010).The molecular variance analysis of xiang yuan population exhibited 73%of the variation within the population.[Conclusion]Citri fructus germplasm resources in China have rich phenotypic genetic variation and high diversity.关键词
香橼/种质资源/表型鉴定/聚类分析/遗传多样性Key words
citri fructus/germplasm resource/phenotypic identification/cluster analysis/genetic diversity引用本文复制引用
魏丽娜,蒋景龙,邓茜茜,李岩,李丽,邓家锐,丁德宽..基于表型性状与SSR标记的香橼种质资源遗传多样性分析[J].生物技术通报,2026,42(4):202-215,14.基金项目
陕西理工大学秦巴生物资源与生态环境重点实验室(培育)"市校共建"科研专项(SXC-2305),陕西理工大学校级项目(SLGXCZXZX2206) （培育）
