生物信息学2026，Vol.24Issue(1)：32-43,12.DOI:10.12113/202405011
食管鳞状细胞癌差异类泛素化调节因子的筛选及生物信息学分析
Screening and bioinformatics analysis of differential ubiquitinization regulators in esophageal squamous cell carcinoma
摘要
Abstract
To investigate differentially expressed class ubiquitin-like modifier(Ub-like)regulatory factors and their potential functions in esophageal squamous cell carcinoma(ESCC)based on public databases,we conducted a study.We obtained one ESCC dataset from the TCGA database for Ub-like regulatory factor differential analysis.We used univariate Cox regression and LASSO regression to screen key genes related to prognosis.We used Cox regression to construct a prognostic model to evaluate the independent prognostic value of the key genes.We used RT-qPCR to initially validate the differential genes in ESCC patients by collecting cancer tissues and adjacent tissues.We used Gene MANIA database to draw a protein-protein interaction(PPI)map for the key genes and performed gene function and pathway enrichment analysis on the network's related genes.We studied the correlation between key genes and immune cell infiltration using the TIMER algorithm.Our study identified two Ub-like regulatory factors,DESI2 and SMC6,that were related to the prognosis of ESCC.The RT-qPCR results showed that DESI2 and SMC6 were highly expressed in ESCC(P＜0.05).The core genes identified by PPI and their functional and enrichment analysis suggested that they may be involved in non-homologous end joining and homologous recombination-accelerated DNA repair.Finally,we found that DESI2 and SMC6 were related to immune cell infiltration using the TIMER algorithm.The results suggest that the expression of DESI2 and SMC6 in ESCC has significant heterogeneity and may be involved in tumor progression,providing theoretical basis for the study of the pathogenesis,clinical diagnosis,and therapeutic targets of ESCC.关键词
食管鳞状细胞癌/类泛素化/免疫细胞浸润/生物信息学Key words
Esophageal squamous cell carcinoma/SUMOylation/Immune cell infiltration/Biological information science分类
医药卫生引用本文复制引用
孙清超,晋伟明,张力为,李少杰,李德生..食管鳞状细胞癌差异类泛素化调节因子的筛选及生物信息学分析[J].生物信息学,2026,24(1):32-43,12.基金项目
新疆维吾尔自治区省部共建中亚高发病成因与防治项目(SKL-HIDCA-2020-SG5). （SKL-HIDCA-2020-SG5）
