生物信息学2026，Vol.24Issue(1)：57-69,13.DOI:10.12113/202409005
中医非药物疗法治疗坐骨神经痛作用机制
Mechanism of non-pharmacological therapy of traditional chinese medicine in treating sciatica
摘要
Abstract
Based on bioinformatics,this study aimed to explore potential molecular mechanism of non-pharmacological therapy of traditional Chinese medicine in treating sciatica,as well as the the miRNA-mRNA regulatory network related to sciatica and the material basis.This study retrieved sciatica-related microarray data from the Gene Expression Omnibus(GEO)database and analyzed differentially expressed genes.Support Vector Machine based Recursive Feature Elimination(SVM-RFE)and Random Forest(RF)algorithms were applied to screen out core genes with high diagnostic performance by combining Receiver Operating Characteristic(ROC)curve and boxplot.Mfuzz was utilized to identify gene clusters with similar change characteristics under non-pharmacotherapy of traditional Chinese medicine in treating sciatica.The Protein-Protein Interaction(PPI)analysis was conducted on the String platform,and the results of PPI data were imported into Cytoscape 3.10.2 software.Core genes were then identified using the cytoHubba plugin.Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG),and Disease Ontology enrichment analyses were performed to obtain the mechanism of non-pharmacological therapy of traditional Chinese medicine in treating sciatica.Using 8 miRNA online databases,this study retrieved the core genes related to sciatica and the core genes in non-pharmacological therapy of traditional Chinese medicine in treating sciatica,and then constructed a critical miRNA-mRNA regulatory network.CEA Cell Adhesion Molecule 4(CEACAM4),kelch like family member 14 Gene(KLHL14),Discoidin CUB And LCCL Domain Containing 1(DCBLD1),Dual Specificity Phosphatase 2(DUSP2),IKAROS Family Zinc Finger 2(IKZF2),and Neuronal Cell Adhesion Molecule Gene(NRCAM)may be biomarkers for the diagnosis of sciatica.Non-pharmacological therapy of traditional Chinese medicine in treating sciatica may be based on fibroblast growth factor 6(FGF6),Peroxisome Proliferator-Activated Receptor Gamma(PPARG),Insulin like growth factor 1(IGF1),Apolipoprotein E(APOE)mediates the Phosphatidylinositide 3-kinases/protein kinase B(PI3K/AKT)signaling pathway and Adenosine 5'-monophosphate-activated protein kinase(AMPK)signaling pathway in treating sciatica.A total of 21 miRNAs,including hsa-mi-27a-3p and hsa-miR-27b-3p,were recruited from the sciatica core gene and the core gene of non-pharmacological therapy of traditional Chinese medicine in treating sciatica.In this study,bioinformatics were used to successfully screen a number of core genes that may be associated with sciatica.At the same time,we also discovered the core genes and mechanism of action of non-pharmacological therapy of traditional Chinese medicine in treating sciatica,which provides a new idea for the treatment of sciatica.Finally,we constructed a miRNA-mRNA regulatory network,which may play an important role in the pathogenesis of sciatica and the therapeutic mechanism of non-pharmacological therapy of traditional Chinese medicine in treating sciatica.关键词
生物信息学/坐骨神经痛/中医非药物疗法/机器学习Key words
Bioinformatics/Sciatica/Non-pharmacological therapy of traditional chinese medicine/Machine learning分类
医药卫生引用本文复制引用
李远志,黄江海,李志文,迟敬轩,方志远..中医非药物疗法治疗坐骨神经痛作用机制[J].生物信息学,2026,24(1):57-69,13.基金项目
北京中医药大学高层次人才科研创业项目(No.2021-XJKYQD-001). （No.2021-XJKYQD-001）
