生物信息学2026，Vol.24Issue(1)：70-84,15.DOI:10.12113/202407007
基于非天然核酸的高密度DNA存储编码方法
High-density DNA storage encoding method based on unnatural nucleic acids
摘要
Abstract
In order to adapt the storage of non-natural nucleic acids(dP,dZ,dS,dB)and natural nucleic acids(A,T,C,G),and to further improve the DNA storage density,this paper proposes a high-density DNA storage coding method based on non-natural nucleic acids.The method firstly designs a dynamic rotation mapping algorithm to control the GC content and homopolymer length,then based on the dynamic rotation mapping algorithm,a heptadecimal Huffman algorithm adapted to octanucleic acid is proposed to enhance the DNA storage density,and finally,the data error correction is realized by utilizing the heptadecimal Hamming code.After utilizing this method to encode files of different formats of text,audio and pictures,the average coding density of this method can reach 3.38 bits/nt,and the content of each base pair can be maintained stably between 23%and 26.5%,and there is no single base repetition.Simulation experiments show that the proposed method improves the DNA storage density,constrains the GC content and homopolymer length and has good error correction capability.关键词
DNA存储/人工核苷酸/哈夫曼编码/映射方法/GC含量约束/均聚物Key words
DNA Storage/Artificial nucleotide/Huffman coding/Mapping method/GC content constraints/Homopolymer分类
信息技术与安全科学引用本文复制引用
刘彦军,杨越飞,胡迎新..基于非天然核酸的高密度DNA存储编码方法[J].生物信息学,2026,24(1):70-84,15.基金项目
河北省教育厅自然基金重点项目(No.ZD2022098). （No.ZD2022098）
