山西农业大学学报（自然科学版）2026，Vol.46Issue(2)：14-21,8.DOI:10.13842/j.cnki.issn1671-8151.202508019
基于宏基因测序的藜麦内生真菌多样性及群落结构分析
Analysis of diversity and community structure of endophytic fungi in quinoa based on metagenomic se-quencing
摘要
Abstract
[Objective]This study aimed to clarify the effects of different varieties,growth stages,and inflorescence types on the diversity of endophytic fungi in quinoa.[Methods]Materials of four varieties at different growth stages were collected from a quinoa cultivation base in Shanxi Province:'Baili 1'(Variety 1),'Baili 2'(Variety 2),'Jinghui 1'(Variety 3),and'Hong-li 2'(Variety 4).Using the metagenomic sequencing method,after quality control of the obtained data,library construction was performed,followed by operational taxonomic units(OTU)clustering analysis,species diversity analysis,and other diver-sity related analyses.[Results]The endophytic fungi in quinoa mainly included 38 genera.The five genera with the highest rela-tive abundance were Alternaria spp.(44.47%),Fusarium spp.(10.23%),Aulographum spp.(9.81%),Aspergillus spp.(6.69%),and Sporormia spp.(5.46%).Differences in the diversity and community structure of endophytic fungi were ob-served among the varieties.Variety 1 contained 32 genera,dominated by Fusarium spp.;Variety 2 contained 17 genera,domi-nated by Rhizopus spp.;Variety 3 and 4,both dominated by Alternaria spp.,contained 34 and 18 genera,respectively.Dif-ferences were also found when inflorescence types were different:the glomerulate type had 34 genera,the intermediate type had 32 genera,and the amaranthiform type had 17 genera.The denser inflorescence types had higher numbers of endophytic fungal genera,while the sparser inflorescence type had fewer.The growth stages also affected endophytic fungal diversity,with 4～9 genera at the flowering stage,10～23 genera at the filling stage,and 10～31 genera at the maturity stage.The num-ber of endophytic fungal genera increased with the increase of growth stages.[Conclusion]Varieties,inflorescence type,and growth stage all influenced the diversity and community structure of endophytic fungi in quinoa.Among these factors,the ef-fect of variety was the most significant,followed by inflorescence type,while growth stage had the least influence.关键词
藜麦/品种/生育期/宏基因组测序/内生真菌Key words
Quinoa/Varietiy/Growth stages/Metagenomic sequencing/Endophytic fungi分类
农业科技引用本文复制引用
王天禧,杨振永,曹瑞鹏,任璐,吕红,秦楠,殷辉,赵晓军..基于宏基因测序的藜麦内生真菌多样性及群落结构分析[J].山西农业大学学报（自然科学版）,2026,46(2):14-21,8.基金项目
山西省重点研发计划(2022ZDYF117) （2022ZDYF117）
山西省现代农业产业技术体系建设专项(2025CYJSTX03-31) （2025CYJSTX03-31）
"特""优"农业高质量发展科技支撑工程项目(TYGC25-01 （）
TYGC25-02) （）
