山西农业大学学报（自然科学版）2026，Vol.46Issue(2)：99-107,9.DOI:10.13842/j.cnki.issn1671-8151.202508033
蝗虫微孢子虫假定黏附基因特性及在东亚飞蝗体内时空表达分析
Characteristics of putative Adhesion gene of Paranosema locustae and its spatiotemporal expression analysis in Locusta migratoria
摘要
Abstract
[Objective]This study aimed to investigate the adhesion target of Paranosema locustae and its mediated toxicity mech-anisms.[Methods]Bioinformatic analysis and quantitative real-time PCR(qPCR)were employed to examine the molecular characteristics and expression patterns of a hypothesized adhesion gene(ADH1)in P.locustae.[Results]The cloned open reading frame of the putative adhesion gene was 885 bp,encoding 294 amino acids with a molecular weight of approximately 32.73 kDa and an isoelectric point of 8.69.Spatiotemporal expression analysis revealed significant temporal and spatial specific-ity:temporally,the ADH1 gene was highly expressed across tissues during both early(3 days post-infection,dpi)and late(12～18 dpi)infection stages,while during the mid-stage(6～9 dpi),expression focused on tissues associated with pathogen colonization and immunity,such as the gonads and Malpighian tubules;spatially,the tissues with high expression dynamically shifted over the course of infection:early expression concentrated in the hindgut,mid-stage expression focused on the gonads,and late-stage expression was also prominent in immune-related tissues such as the fat body and Malpighian tubules.Notably,in all highly expressed tissues,the transcriptional peak of ADH1 preceded the increase in P.locustae pathogen load.[Conclu-sion]It was confirmed that ADH1 played a critical role in the infection of host cells by P.locustae,and that adhesion may occur prior to spore activation and host cell infection.This study provided a new perspective into the infection mechanism of P.locus-tae and laid a foundation for further research into the pathogenic process.关键词
蝗虫微孢子虫/假定黏附基因/ADH1/生物信息学分析/时空表达Key words
P.locustae/Putative Adhesion gene/ADH1/Bioinformatics analysis/Spatiotemporal expression分类
生物科学引用本文复制引用
康晗晔,郭玮琦,霍帆,张航悦,张娜,季荣,扈鸿霞..蝗虫微孢子虫假定黏附基因特性及在东亚飞蝗体内时空表达分析[J].山西农业大学学报（自然科学版）,2026,46(2):99-107,9.基金项目
新疆维吾尔自治区自然科学基金面上项目(2023D01A45) （2023D01A45）
新疆维吾尔自治区"三农"骨干人才培养项目(2024SNGGGCC039) （2024SNGGGCC039）
天山英才领军人才项目(TSYCLJ0016) （TSYCLJ0016）
