山西医科大学学报2026，Vol.57Issue(3)：267-275,9.DOI:10.13753/j.issn.1007-6611.2026.03.004
和胃保津汤通过SCF/c-Kit与MEK/ERK通路改善糖尿病胃轻瘫
Hewei Baojin decoction allviates diabetic gastroparesis via SCF/c-Kit and MEK/ERK signaling pathways
摘要
Abstract
Objective To explore the efficacy and mechanism of Hewei Baojin decoction in diabetic gastroparesis(DGP)rats.Methods Sixty male Wistar rats were randomly divided into control group,model group,Mosapride group(1.35 mg/kg)and Hewei Baojin decoction low-,medium-and high-dose(0.05,0.10,0.20 mL/kg)groups(n=10 per group).Except for control group,the rats in the other five groups were treated with streptozotocin combined with an irregular high-fat and high-sugar diet to induce DGP model.Rats in Mosapride group were administered 1.35 mg/kg Mosapride by gavage,while those in Hewei Baojin decoction low-,medium-,and high-dose groups were administered the corresponding doses of the decoction by gavage,respectively.After 8 weeks of administration,body weight,fasting blood glucose and gastric emptying rate were measured.The levels of motilin(MTL)and gastrin(GAS)in gastric antrum tissue were detected by ELISA.Histopathological changes of gastric antrum tissues were observed by HE staining,and gastric antral cell apoptosis was observed by TUNEL staining.The positive expressions of stem cell factor(SCF),stem cell factor receptor(c-Kit),phosphorylated mitogen-activated protein kinase(p-MEK),and phosphorylated extracellular signal-regulated kinase(p-ERK)in gastric antrum tissue were detected by immunohistochemistry,and the protein expression levels of SCF,c-Kit,p-MEK,MEK1/2,p-ERK,and ERK1/2 in gastric antrum tissue were quantified by Western blot.Results Compared with control group,rats in model group exhibited decreased body weight,gastric emptying rate,MTL and GAS levels,along with increased fasting blood glucose,significant gastric antrum tissue damage,elevated apoptosis rate,reduced positive expression of SCF,c-Kit,p-MEK,and p-ERK,and down-regulated protein expres-sion levels of SCF,c-Kit,p-MEK/MEK1/2,and p-ERK/ERK1/2(all P<0.05).Compared with model group,the rats in Mosapride group and Hewei Baojin Decoction groups showed increased body weight,gastric emptying rate,MTL and GAS levels,along with decreased fasting blood glucose,ameliorated gastric antrum tissue damage,reduced apoptosis,enhanced positive expression of SCF,c-Kit,p-MEK,and p-ERK,and up-regulated protein expression levels of SCF,c-Kit,p-MEK/MEK1/2,and p-ERK/ERK1/2(all P<0.05).Furthermore,the effects of Hewei Baojin decoction group were observed in a dose-dependent manner.Conclusion Hewei Baojin decoc-tion can improve the gastrointestinal function in DGP rats through modulation of the SCF/c-Kit and MEK/ERK pathways.关键词
糖尿病胃轻瘫/和胃保津汤/胃窦组织/大鼠/成纤维细胞生长因子/丝裂原激活蛋白激酶激酶Key words
diabetic gastroparesis/Hewei Baojin decoction/gastric antrum tissue/rats/fibroblast growth factor/mitogen-activated protein kinase kinase分类
医药卫生引用本文复制引用
贺红梅,邱玉萍,王芳,王齐有,邓德强..和胃保津汤通过SCF/c-Kit与MEK/ERK通路改善糖尿病胃轻瘫[J].山西医科大学学报,2026,57(3):267-275,9.基金项目
乌鲁木齐市中医药科技创新项目(ZYYMS-51) （ZYYMS-51）
新疆维吾尔自治区卫生健康委员会"天山英才"医药卫生高层次人才培养计划项目(TSYC202301B167) （TSYC202301B167）
