实用医学杂志2026，Vol.42Issue(8)：1407-1414,8.DOI:10.3969/j.issn.1006-5725.2026.08.014
Sugen低氧诱导肺动脉高压小鼠模型中免疫细胞亚群的动态变化
Dynamic changes of immune cell subsets in a mouse model of Sugen-hypoxia-induced pulmonary hypertension
摘要
Abstract
Objective To investigate the dynamic changes of immune cell subsets in a mouse model of pulmonary hypertension(PH)induced by Sugen-hypoxia(SuHx).Methods A SuHx-PH mouse model was established through intraperitoneal injection of Sugen 5416,followed by exposure to hypoxia.Mouse lung tissues were collected prior to modeling and at 1 week(h1w),2 weeks(h2w),3 weeks(h3w),and 4 weeks(h4w)post-modeling.Flow cytometry was employed to detect the proportional alterations of immune cell subsets(including T cells,B cells,macrophages,eosinophils(EOS),neutrophils(Neu),natural killer(NK)cells,and innate lymphoid cells(ILCs)within CD45+immune cells.Results Compared with the control group,the immune cell subsets in SuHx model mice demonstrated significant temporal changes.Among these,the total CD11c+F4/80+macrophages and interstitial macrophages(IM,SIGLECF-)showed a significant decreasing tendency as the modeling time progressed;the cell levels of alveolar macrophages(AM,SIGLECF+)continuously increased(P＜0.05);EOS,ILCs,Neu,and CD4+T cells displayed a significant fluctuating pattern,with significant differences between groups(P＜0.05).Conclusions The proportions of immune cell subsets in the lung tissues of SuHx-PH mice exhibit significant temporal changes,indicating that different immune cells may play stage-specific roles in the progression of PH.This study offers a foundation for a more in-depth understanding of the immunoinflammatory mechanism of PH and the identification of potential immune intervention targets.关键词
Sugen低氧模型/肺动脉高压/免疫细胞/流式细胞术Key words
Sugen-hypoxia model/pulmonary hypertension/immune cells/flow cytometry分类
医药卫生引用本文复制引用
刘杰,贾玉峰,崔烨,王炜,孙英,王蕾,薛鑫,郭煜,高振强,张寒晓,张牧之,隆寰宇,吕佳璐,许蒙雨..Sugen低氧诱导肺动脉高压小鼠模型中免疫细胞亚群的动态变化[J].实用医学杂志,2026,42(8):1407-1414,8.基金项目
国家自然科学基金项目(编号:82202014) （编号:82202014）
北京市教育委员会科研计划一般项目(编号:KM202310025029) （编号:KM202310025029）
临港实验室"求索杰出青年计划"开放课题(编号:LG-QS-202205-08) （编号:LG-QS-202205-08）
