时珍国医国药2026，Vol.37Issue(7)：1343-1350,8.DOI:10.70976/j.1008-0805.SZGYGY-2026-0721
电针通过激活肠道FXR/FGF15通路改善糖尿病心肌病小鼠脂质代谢的机制研究
Mechanism of electroacupuncture in improving lipid metabolism in diabetic cardiomyopathy mice via activation of the intestinal FXR/FGF15 pathway
摘要
Abstract
Objective To investigate the effects of electroacupuncture on the intestinal farnesoid X receptor(FXR)/fibroblast growth factor 15(FGF15)bile acid signaling pathway in mice with diabetic cardiomyopathy(DCM)and to explore the underlying mechanism by which electroacupuncture(EA)improves lipid metabolism.Methods C57BL/6J mice were randomly divided into a control group,model group,EA group,EA plus inhibitor group,and agonist group(n=8 per group).Except for the control group,DCM models were established by streptozotocin injection combined with a high-fat diet.The EA group received stimulation at Zhongwan(CV/12),Guanyuan(CV/4),Zusanli(ST/36),and Neiguan(PC/6)for 10 weeks.The EA plus inhibitor group was additionally administered the FXR inhibitor Z-Guggulsterone,while the agonist group received the FXR agonist GW4046.Body weight,fasting blood glucose(FBG),lipid profiles,and markers of myocardial injury,oxidative stress,and inflammation were measured.Histopathological changes in myocardial and ileal tissues were examined by HE staining.Protein and mRNA expression levels of FXR and FGF15 in ileal tissue were assessed by Western blot(WB)and real-time quantitative PCR.Results Compared with the control group,the model group showed significantly increased FBG,serum TC,TG,and LDL-C levels,decreased HDL-C and body weight,aggravated myocardial and ileal tissue damage,and downregulated FXR and FGF15 expression(P<0.05).Compared with the model group,these abnormalities were significantly improved in the EA and agonist groups,accompanied by alleviated histopathological injury and upregulated FXR and FGF15 expression(P<0.05).The protective effects of EA were markedly attenuated by FXR inhibition.Conclusion EA may alleviate lipid metabolism disorders and myocardial injury in DCM mice by activating the intestinal FXR/FGF15 signaling pathway.关键词
糖尿病心肌病/电针/脂代谢/法尼酯X受体/FXR/FGF15信号通路Key words
Diabetic cardiomyopathy/Electroacupuncture/Lipid metabolism/Farnesoid X receptor(FXR)/FXR/FGF15 sig-naling pathway分类
医药卫生引用本文复制引用
余彦欣,王巧芸,苑亚涛,罗亦炫,周春阳,张艳佶,黄伟..电针通过激活肠道FXR/FGF15通路改善糖尿病心肌病小鼠脂质代谢的机制研究[J].时珍国医国药,2026,37(7):1343-1350,8.基金项目
湖北省自然科学联合基金(2022CFD024 （）
2025AFD520) （）
湖北中医药大学"双一流"建设重点类专项科研项目(2023ZZXT005) （2023ZZXT005）
湖北省技术创新计划重点研发项目(2025BCB024) （2025BCB024）
湖北省时珍人才工程项目(鄂卫函[2024]256号) （鄂卫函[2024]256号）
湖北省中医药管理局中医药科研项目(ZY2025Q040 （）
ZY2025D001) （）
