特产研究2026，Vol.48Issue(2)：35-43,9.DOI:10.16720/j.cnki.tcyj.2024.189
壳寡糖联合丛枝菌根真菌对烟草根结线虫病的防治评价
Evaluation of Chitooligosaccharide Combined with Arbuscular Mycorrhi-zal Fungi against on Tobacco Root-knot Nematode Disease
摘要
Abstract
Tobacco root knot nematode disease caused by tobacco root-knot nematode(RKN)has a widespread and difficult to control,which restricts the cultivation and production of tobacco.To explore the mechanism of co-application of AMF and chitooligosaccharide in improving tobacco resistance to RKN,this study researched the changes in tobacco agronomic traits,root vigor,and key enzyme activity,microbial community composition of rhizospheric soil after inoculation of RKN 40 days which under the effects of chitooligosaccharide and AMF.Meanwhile,the resistance index of tobacco to RKN and prevention rate were analyzed statistically.Results showed that the co-appli-cation of AMF and chitooligosaccharide effectively reduced the number of RKN,egg number,reproduction index,and root knot index,the reproductive index and the root knot index,which decreased by 44.5％,87.8％,89.3％and 79.5％respectively compared with the control group,which significantly increased the root vigor of tobacco.In addition,the co-application of AMF and chitooligosaccharide also incre-ased soil enzyme activity and the abundance of beneficial microorganisms in rhizosphere soil,compared with control group,the activities of soil dehydrogenase,sucrase,cellulase and polyphenol oxidase were increased to 118.41％,41.62％,44.06％and 31.86％,also can increase the contents of nicotine,total sugar and potassiumby 11.4％,17.5％and 24.0％,respectively,further improved the effective resistance of to-bacco to RKN.The results indicated that chitooligosaccharide and AMF could significantly reduce the incidence of RKN and improve the qual-ity of tobacco leaves,and provided a theoretical basis and solution for alleviating the damage of root-knot nematode to tobacco growth.关键词
烟草根结线虫病/壳寡糖/丛枝菌根真菌/根系微生态Key words
tobacco root-knot nematode disease/chitooligosaccharides/arbuscular mycorrhizal fungi/root microecology分类
农业科技引用本文复制引用
胡志明,赵忠利,丁立和,孙加利,荆常亮,尹兴盛,濮永瑜,戴永平,马俊卿,杨霞,胡希好,王树键,李强..壳寡糖联合丛枝菌根真菌对烟草根结线虫病的防治评价[J].特产研究,2026,48(2):35-43,9.基金项目
云南省烟草公司科技计划项目(2021530000242012) （2021530000242012）
