天津农业科学2026，Vol.32Issue(3)：9-17,9.DOI:10.3969/j.issn.1006-6500.2026.03.002
多肉植物'柠檬玫瑰'及其嵌合体组培体系的建立
Establishment of Tissue Culture System of Echeveria'Lemon Rose'and Its Chimeras
摘要
Abstract
In order to establish the tissue culture and rapid propagation system of succulent plants Echeveria'Lemon Rose'and its chimera(Echeveria'Lemon Rose'prol.Variegata),the leaves of Echeveria'Lemon Rose'and Echeveria'Lemon Rose'prol.Variegata were used as explants to screen the best disinfection methods.The effects of different concentrations of NAA and 6-BA on adventitious bud induction,proliferation and rooting were discussed.The results showed that the optimal disinfection combination of Echeveria'Lemon Rose'was 75%alcohol soaking for 30 s and 0.5%NaClO soaking for 15 min,and the sterilization rate could reach 93.41%.The optimum disinfection combination of Echeveria'Lemon Rose'prol.Variegata was 75%alcohol soaking for 30 s and 0.5%NaClO solution soaking for 12 min,and the sterilization rate reached 97.07%.The optimum medium for adventitious bud induction of Echeve-ria'Lemon Rose'and Echeveria'Lemon Rose'prol.Variegata was MS+1 mg·L-1 6-BA+0.3 mg·L-1 NAA,and the adventitious bud in-duction rates were 85.43%and 75.33%,respectively.The brocade rate of Echeveria'Lemon Rose'prol.Variegata also reached of 26.00%.The optimum formula of Echeveria'Lemon Rose'proliferation medium was MS+3 mg·L-1 6-BA+0.2 mg·L-1 NAA,and the prolifer-ation coefficient was 8.56.The optimum proliferation medium formula of Echeveria'Lemon Rose'prol.Variegata was MS+3 mg·L-1 6-BA+0.1 mg·L-1 NAA,and the proliferation coefficient was 11.4,the brocade rate reached of 97.50%.The optimum formula for rooting and strong seedling medium of Echeveria'Lemon Rose'and its chimera was 1/2MS without adding hormones.The rooting rate of both reached 100%.The number of days after opening the bottle for transplanting of Echeveria'Lemon Rose'and Echeveria'Lemon Rose'prol.Variegata were 3 d.The survival rate of Echeveria'Lemon Rose'was 85.79%,and that of Echeveria'Lemon Rose'prol.Variegata was 81.16%.In summary,this study provides a theoretical basis for the rapid propagation of Echeveria'Lemon Rose'and Echeveria'Lemon Rose'prol.Variegata,which is of great significance to its industrialization development.关键词
多肉植物/'柠檬玫瑰'/嵌合体/组织培养Key words
succulent flowers/Echeveria'Lemon Rose'/chimera/tissue culture分类
农业科技引用本文复制引用
王杰,李林芡,张曼曼,毕作斌,李记锋,孙宪芝..多肉植物'柠檬玫瑰'及其嵌合体组培体系的建立[J].天津农业科学,2026,32(3):9-17,9.基金项目
淄博润邦生态农业科技发展有限公司科技支撑项目(ZBRB202101) （ZBRB202101）
