天津科技大学学报2026，Vol.41Issue(2)：1-10,10.DOI:10.13364/j.issn.1672-6510.20240190
裂解性多糖单加氧酶的生物信息学分析
Bioinformatic Analysis of the Lytic Polysaccharide Monooxygenases
摘要
Abstract
Lytic polysaccharide monooxygenase(LPMO)can catalyze the oxidative lysis of glycosidic bonds in polysaccha-ride such as cellulose,and plays a key role in the degradation of biomass such as straw.To further explore the structure-activity relationship of LPMO,115 experimentally verified LPMO included in UniProtKB database were systematically stud-ied using bioinformatics analysis technology.According to its source statistics,101 LPMO from AA9 family accounted for as high as 87.8%.Further molecular evolutionary tree analysis based on genetic distance and cluster relationship obtained six groups,all AA9 family LPMO were distributed in Group I-Group V and the other three families were clustered in Group Ⅵ.Analysis of 34 LPMO derived from Aspergillus revealed that all of these proteins had at least two copper ion binding sites and contained five 100%highly conserved sequence sites.Further study of the motifs and domains of LPMO from Aspergil-lus showed that LPMO with highly similar motifs composition were close in evolution and development,but the different order of motifs can also affect the catalytic activity;the catalytic domain of LPMO of AA9 family mainly had cellulolytic enzyme activity,while the catalytic domain of AA13 family mainly had starch degradation activity.This study lays a theo-retical foundation for further investigation of LPMO structure-activity relationship.关键词
裂解性多糖单加氧酶/生物信息学分析/分子进化/结构域Key words
lytic polysaccharide monooxygenase/bioinformatics analysis/molecular evolution/domain分类
生物科学引用本文复制引用
白琳,丁灿灿,王富豪,路福平,刘夫锋..裂解性多糖单加氧酶的生物信息学分析[J].天津科技大学学报,2026,41(2):1-10,10.基金项目
国家重点研发计划项目(2021YFC2102700) （2021YFC2102700）
国家自然科学基金项目(32272269) （32272269）
天津市科技重大专项与工程项目(25ZXWCSY00160) （25ZXWCSY00160）
