西北农业学报2026，Vol.35Issue(4)：662-670,9.DOI:10.7606/j.issn.1004-1389.2026.04.006
高温胁迫对小麦花药细胞程序化死亡的影响及TaMCA4基因表达分析
Effects of High-Temperature Stress on Programmed Cell Death in Wheat Anthers and TaMCA4 Gene Expression
摘要
Abstract
High-temperature is a major environmental stress factor affecting wheat growth and devel-opment.To investigate the regulatory mechanisms of programmed cell death(PCD)occurring during high-temperature induced anther abortion,anthers at different developmental stages(late-uninucleate stage,binucleate stage,and trinucleate stage)were collected from wheat grown under control and high-temperature stress conditions.Morphological changes in the tapetum and DNA fragmentation were comparatively analyzed between treatments.In addition,the expression patterns of the PCD-re-lated gene TaMCA4 were also investigated.The results showed that under high-temperature stress,the cytoplasmic density of the tapetum began to diminish from the late-uninucleate stage.By the tri-nucleate stage,the tapetum had completely degraded,indicating the premature degradation of the ta-petal cells.This process was accompanied by a characteristic DNA laddering pattern,a hallmark of PCD.During anther development,the expression of TaMCA4 was suppressed at the late uninucleate stage under high-temperature stress,resulting in relatively low expression levels.However,TaM-CA4 expression was significantly upregulated at binucleate and trinucleate stages,suggesting its in-volvement in the PCD process during high-temperature stress induced anther abortion.Protein-protein interaction analysis predicted ten proteins interacting with TaMCA4,among which the glycosyltrans-ferase family protein A0A0R6UPC9 exhibited the highest interaction score.These results may provide new insights into the molecular mechanisms of high-temperature induced anther abortion in wheat.关键词
小麦/花药/高温胁迫/细胞程序化死亡/TaMCA4Key words
Wheat/Anthers/High-temperature stress/Programmed cell death/TaMCA4引用本文复制引用
赵丽丽,刘易科,朱展望,王书平,金汉炳,马驰,冯佳宜,尹晓康,蓝罗冰,屈杭丽,张迎新,方正武..高温胁迫对小麦花药细胞程序化死亡的影响及TaMCA4基因表达分析[J].西北农业学报,2026,35(4):662-670,9.基金项目
国家重点研发计划(2024YFD1201103) （2024YFD1201103）
湖北省重点研发计划(2024BBB004) （2024BBB004）
粮食作物种质创新与遗传改良湖北省重点实验室开放课题(2024lzjj15). National Key Research and Development Program(No.2024YFD1201103) （2024lzjj15）
Key Re-search and Development Program of Hubei Province(No.2024BBB004) （No.2024BBB004）
Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement(No.2024lzjj15). （No.2024lzjj15）
