西北农业学报2026，Vol.35Issue(4)：707-713,7.DOI:10.7606/j.issn.1004-1389.2026.04.011
野生西藏沙棘多功能菌株的筛选鉴定及促生能力比较
Screening,Identification and Comparative Analysis of Growth-promoting Abilities of Multifunctional Bacterial Strains Isolated from Hippophae thibetana Schlechtend
摘要
Abstract
In this study,rhizosphere soil samples of Hippophae thibetana Schlechtend(Wild Tibetan Seabuckthorn)from Qinghai Province were collected to isolate,purify,and classify microbial strains.The functional abilities of the strains,including nitrogen fixation,phosphorus solubilization and po-tassium release,were evaluated.The growth-promoting effects of selected multifunctional strains were also assessed.The results showed that three strains of Pseudomonas(N7,W4,N19)and one strain of Enterohepater(Y65)were identified.After five days of cultivation,the diameter of the nitro-gen-fixing halo ranged from 9.07 to 11.63 mm,and nitrogenase content ranged from 183.41 to 241.38 U/L.The diameter of the phosphorus-solubilizing halo ranged from 6.80 to 16.43 mm,with available phosphorus content ranging from 13.17 to 18.77 μg/mL.The diameter of the potassium-re-leasing zone ranged from 14.43 to 19.41 mm,and available potassium content ranged from 18.47 to 21.47 μg/mL.The results of the growth-promoting test showed that all four strains increased the ger-mination rate of lettuce,with the germination rates ranging from 78.89％to 90.00％.All strains ex-hibited significant abilities of nitrogen fixation,phosphorus solubilization,potassium release,and growth promotion.Among them,strain Y65 showed the most significant effect on biomass accumula-tion.关键词
西藏沙棘/假单胞菌/根际促生菌/促生能力Key words
Hippophae thibetana Schlechtend/Plant growth-promoting rhizobacteria(PGPR)/Growth-promoting ability引用本文复制引用
三鸿源,郭琳,李宗德,吴威泰,马玉花..野生西藏沙棘多功能菌株的筛选鉴定及促生能力比较[J].西北农业学报,2026,35(4):707-713,7.基金项目
青海省科技厅基础研究计划项目(2024-ZJ-941) （2024-ZJ-941）
青海省"昆仑英才·高端创新创业人才"计划项目(2022). Funding was Provided by the Basic Research Program of the Qinghai Provincial De-partment of Science and Technology(No.2024-ZJ-941) （2022）
the"Kunlun Talent-High-End Innovation and Entrepreneurship Talent"Program of Qinghai Province(No.2022). （No.2022）
