枣疯病枣树内生细菌多样性分析及生防菌筛选鉴定
Analysis of Endophytic Bacteria Diversity in Jujube Trees with Jujube Witches Broom and Screening and Identification of Biocontrol Strains
摘要
Abstract
Jujube witches broom disease,caused by phytoplasma,is a destructive plant disease affect-ing jujube trees.Studying the diversity of endophytic bacteria in infected trees and screening for po-tential biocontrol strains can provide insights into phytoplasma infection mechanisms and support the development of biological control strategies.In this study,roots and branches of both healthy and phytoplasma-infected jujube trees were analyzed using high-throughput sequencing to assess endophyt-ic bacterial diversity.Traditional isolation methods were combined with GFP fluorescence labeling to screen and validate potential biocontrol strains.The results showed that:(1)Compared with healthy trees,phytoplasma-infected jujube trees exhibited increased endophytic bacterial diversity in bran-ches,along with a higher relative abundance of Bacillus spp.In contrast,root endophytic bacterial diversity decreased,with a reduced relative abundance of Pseudomonas spp.(2)Fifteen endophytic bacterial strains were isolated from the roots of healthy trees.Among them,Pseudomonas sp.L2 stably colonized tissue-cultured jujube seedlings.When applied to phytoplasma-infected seedlings,strain L2 significantly increased plant height,fresh mass,and dry mass.In conclusion,phytoplasma infection reduces the diversity of endophytic bacteria in jujube roots,particularly diminishing the abundance of Pseudomonas spp.Moreover,the endophytic strain Pseudomonas sp.L2 alleviates dis-ease symptoms and shows potential as a biocontrol agent against jujube witches' broom disease.These findings lay the foundation for developing L2-based biocontrol formulations and advancing dis-ease management strategies.关键词
枣疯病/植原体/高通量测序/多样性/生物防治Key words
Jujube witches broom/Phytoplasma/High-throughput sequencing/Diversity/Biological control引用本文复制引用
林金水,李兰,屈晓逸,朱旭飞,韩嘉仪,成娟丽,张向前..枣疯病枣树内生细菌多样性分析及生防菌筛选鉴定[J].西北农业学报,2026,35(4):736-748,13.基金项目
国家自然科学基金(32070103) （32070103）
陕西省自然科学基础研究计划(2021JM-415) （2021JM-415）
作物抗逆与高效生产全国重点实验室开放课题(SKLCSRHPKF12) （SKLCSRHPKF12）
陕西省"特支计划"区域发展人才项目(2020-44) （2020-44）
陕西省普通高等学校青年杰出人才支持计划(2018-111) （2018-111）
陕西高校青年创新团队(2022-943) （2022-943）
延安市科技计划(2024-JBZ-001) （2024-JBZ-001）
延安大学科研计划(2023HBZ-001,2023CGZH-007) （2023HBZ-001,2023CGZH-007）
陕西省大学生创新创业训练项目(S202310719142). National Natural Science Foundation of China(No.32070103) （S202310719142）
Natural Science Foundation of Shaanxi Province(No.2021JM-415) （No.2021JM-415）
Open Project of National Key Laboratory of Crop Stress and Efficient Production(No.SKLCSRHPKF12) （No.SKLCSRHPKF12）
Special Support Program for Regional De-velopment Talents of Shaanxi Province(No.2020-44) （No.2020-44）
Youth Outstanding Talent Support Program of Shaanxi Province(No.2018-111) （No.2018-111）
Youth Innovation Team of Shaanxi Universities(No.2022-943) （No.2022-943）
Yan'an Science and Technology Plan(No.2024-JBZ-001) （No.2024-JBZ-001）
Yan'an University Research Plan(No.2023HBZ-001,No.2023CGZH-007) （No.2023HBZ-001,No.2023CGZH-007）
Innovation and Entrepreneurship Training Program for College Students of Shaanxi Province(No.S202310719142). （No.S202310719142）
