现代中西医结合杂志2026，Vol.35Issue(4)：439-447,9.DOI:10.3969/j.issn.1008-8849.2026.04.001
固肾泄浊和络方对糖尿病肾病大鼠肾脏的保护作用及对NF-κB/Wnt/β-catenin信号通路的影响
Protective effect of Gushen Xiezhuo Heluo prescription on the kidney of diabetic kidney disease rats and its influence on NF-κB/Wnt/β-catenin pathway
摘要
Abstract
Objective It is to investigate the protective effect of Gushen Xiezhuo Heluo prescription on the kidney of rats with diabetic kidney disease and its effect on nuclear factor kappa-B(NF-κB)/Wnt/β-catenin pathways.Methods Seventy-two male SD rats were taken,12 of which were randomly selected as normal group,and the remaining 60 rats were given 1%streptozotocin(STZ)solution by a single intraperitoneal injection into the left side to establish models of diabetic kidney disease.50 successfully model rats were randomly divided into model group,irbesartan group,low-dose,medium-dose and high-dose groups of Gushen Xiezhuo Heluo prescription,with 10 rats in each group.The irbesartan group was ad-ministered irbesartan via gastric gavage at a dose of 0.0781 g/kg,while the low-dose,medium-dose and high-dose groups of Gushen Xiezhuo Heluo prescription were administered the formula solution via gastric gavage at concentrations of 0.53 g/mL,1.06 g/mL,and 2.11 g/mL,respectively,the control group and normal group were administered normal saline via gavage,all once daily,continuously treated for 6 weeks.The general condition of the rats was observed,their body weight,random blood glucose,and 24-hour urine protein(24hUP)were measured before the first gavage and after the final gavage,the se-rum levels of creatinine(SCr)and blood urea nitrogen(BUN)were measured after the final gavage;the renal tissue pa-thology was observed by HE staining and PAS staining;the mRNA and protein expressions of NF-κB,type Ⅳ collagen,Wnt4,β-catenin,matrix metalloproteinase(MMP)-7,and MMP-3 in renal tissue were detected by qPCR and Western blot assays,respectively.Results Compared with the normal group,the body weight of rats in the model group was decreased(P<0.05),their random blood glucose,SCr,BUN and 24hUP were significantly increased(all P<0.05);the patholog-ical changes such as thickened basement membrane,increased mesangial matrix,infiltration of interstitial cells and renal fi-brosis were found;the mRNA and protein expressions of NF-κB,type Ⅳ collagen,Wnt4,β-catenin,MMP-7 and MMP-3 in renal tissue were increased(all P<0.05).Compared with the model group,the body weights of rats in the medium-dose and high-dose groups of Gushen Xiezhuo Heluo prescription and irbesartan group were decreased(all P<0.05),their random blood glucose,SCr,BUN and 24hUP were significantly decreased(all P<0.05);the mesangial matrix was de-creased,the infiltration of interstitial cells and renal fibrosis were alleviated;the mRNA and protein expressions of NF-κB,type Ⅳ collagen,Wnt4,β-catenin,MMP-7 and MMP-3 in renal tissue were decreased(all P<0.05),and the improve-ments of these indicators except for the body weight,random glucose,SCr and BUN in the high-dose group of Gushen Xiezhuo Heluo prescription were more significant than those in the other medicated groups(all P<0.05).Conclusion Gushen Xiezhuo Heluo prescription can significantly alleviate renal dysfunction and renal fibrosis,and inhibit inflammatory responses in rats with diabetic nephropathy.It may exert its nephroprotective effects via regulating the NF-κB/Wnt/β-cate-nin signaling pathway,down-regulating the expressions of MMP-7 and MMP-3,and reducing the excessive deposition of type Ⅳ collagen.关键词
糖尿病肾病/固肾泄浊和络方/核因子-κB/Wnt/β-连环蛋白通路/基质金属蛋白酶/Ⅳ型胶原Key words
diabetic kidney disease/Gushen Xiezhuo Heluo prescription/NF-κB/Wnt/β-catenin pathway/MMP-7/MMP-3/type Ⅳ collagen分类
医药卫生引用本文复制引用
徐聪,郑艳辉,王沛然..固肾泄浊和络方对糖尿病肾病大鼠肾脏的保护作用及对NF-κB/Wnt/β-catenin信号通路的影响[J].现代中西医结合杂志,2026,35(4):439-447,9.基金项目
郑艳辉名中医工作室建设项目(2023-NJSMZYGZS-ZYH) （2023-NJSMZYGZS-ZYH）
南京中医药大学临床专病研究院培育项目(LCZBYJYZZ2024-004) （LCZBYJYZZ2024-004）
南京中医药大学自然科学基金项目(XZR2021049) （XZR2021049）
