新疆农业科学2026，Vol.63Issue(1)：161-171,11.DOI:10.6048/j.issn.1001-4330.2026.01.015
薰衣草种子内生细菌T2-29的鉴定与生防促生效果分析
Identification and plant growth promotion of endophyte bacterium T2-29 in lavender seeds
摘要
Abstract
[Objective]This study aims to isolate endophytic bacteria from lavender seeds cultivated in Yili,Xinjiang province,with a focus on screening for strains exhibiting both antagonistic properties against plant pathogens and plant growth-promoting properties.[Methods]Employing tissue homogeniza-tion method,we isolated endophytic bacteria from lavender seeds.We then identified strains that displayed antagonism towards plant pathogenic fungi and growth-promoting effects on maize using plate standoff and selective medium methods.The strains were identified through morphological,physiological,and biochemical characteristics,complemented by the phylogeny of 16S rRNA gene sequences.The impact of these bacteria on maize seed germination and seedling growth was evaluated.[Results]A total of 85 endophytic bacterial strains were isolated from lavender seeds.Strain T2-29,identified as Paenibacillus polymyxa,demonstrated strong inhibitory effects against six plant pathogenic fungi,including Fusarium oxysporum,the causal agent of melon wilt.This strain also showed capabilities in solubilizing phytate phosphorus and potassium,as well as producing protease,cellulase,siderophore and indole-3-acetic acid(IAA).Strain T2-29 solubilized 3.19 mg/L of organic phosphorus and produced 5.42 mg/L of IAA,with siderophore UP values of 3.63,protease UP values of 8.04,and cellulase UP values of 8.30.Inoculation with strain T2-29 led to a 3.7％increase in maize seed germination rate and significantly enhanced the growth of maize seedlings.The length of maize stems,above-ground fresh weight,underground fresh weight,above-ground dry weight,and underground dry weight increased by 30.3％,32.5％,37.4％,21.1％,and 9.9％,respectively.Moreover,the root length,root surface area,root system volume,and average root diameter of maize plants inoculated with T2-29 increased by 11.0％,11.7％,11.6％,and 1.6％,respectively.[Conclusion]The endophytic bacterium T2-29,isolated from lavender seeds,is an exceptional strain with both antagonistic and plant growth-promoting functions.It not only stimulates the germination and growth of maize but also serves as a high-quality microbial resource and provides a theoretical foundation for the development and application of microbial fertilizers.关键词
薰衣草种子/内生菌/菌株鉴定/促生作用/生防作用Key words
lavender seeds/endophytic bacteria/identification/plant growth promotion/biocontrol effects分类
农业科技引用本文复制引用
王君竹,李桂花,张洒洒,柳雯雯,王琛,郭岩彬,焦子伟..薰衣草种子内生细菌T2-29的鉴定与生防促生效果分析[J].新疆农业科学,2026,63(1):161-171,11.基金项目
伊犁州科技计划项目(YZ2023A05) （YZ2023A05）
新疆维吾尔自治区重点研发项目(2022B02021-2) （2022B02021-2）
新疆维吾尔自治区三农骨干人才项目(2023SNGGGCC015) （2023SNGGGCC015）
国家自然科学基金项目(32160312) Yili State Science and Technology Program Project(YZ2023A05) （32160312）
Xinjiang Uygur Autonomous Region Key Research and Development Program Project(2022B02021-2) （2022B02021-2）
Xinjiang Uygur Autonomous Region Sannong Backbone Talent Project(2023SNGGGGGCC015) （2023SNGGGGGCC015）
National Natural Science Foundation of China Project(32160312) （32160312）
