畜牧与兽医2026，Vol.58Issue(5)：1-9,9.
新西兰兔发情与结肠微生物关联性研究
Study on the association between estrus in New Zealand rabbits and microbiota in the colon
摘要
Abstract
The aim of this study was to investigate the relationship between estrogen related to estrus and gut microbiota in New Zealand white rabbits.Female rabbits in diestrus(UN)and estrus(ES)were selected by visual estrus detection based on vulval changes,plasma es-tradiol was measured by enzyme-linked immunosorbent assay(ELISA),and metagenomic detection methods were used to determine the composition and function of colonic microorganisms and estrus status,and the correlation between them was analyzed.The result were as fol-lows:After conducting species annotation and functional analysis on the samples through metagenomic sequencing,it was found that the abundance of the colonic microbiota in New Zealand white rabbits UN was significantly higher than that in ES(P＜0.05).Among them,Al-phaproteobacteria was the representative species of the ES group,Pseudoflavonifractor and Acetatifactor muris were the representative species of the UN group.The KEGG functional difference analysis showed that the abundance of microorganisms related to amino acid metabolism and carbohydrate metabolism was higher in the UN group,while the relative abundance of glycan biosynthesis and metabolism,cell growth and death,and signal transduction was higher in the ES group.The difference analysis of CAZy showed that glycoside hydrolases were significant-ly enriched in the UN group(P＜0.05),while polysaccharide lyase,glycoesterase,and oxidoreductases were significantly enriched in the ES group(P＜0.05).The correlation analysis showed that colonic microbial composition and function were significantly correlated with estrous status and plasma E2 levels(P＜0.05).In summary,this study preliminarily demonstrated that there were significant differences in colonic microbial composition in different estrus states,which might serve as a direction for further research on the mechanism of estrus in New Zeal-and white rabbits.关键词
新西兰白兔/发情期/间情期/结肠微生物Key words
New Zealand white rabbit/estrus/diestrus/colonic microorganism分类
农业科技引用本文复制引用
张洺瑞,张馨月,陈思齐,顾明珂,姜幸慧,陈晶,甘乾福..新西兰兔发情与结肠微生物关联性研究[J].畜牧与兽医,2026,58(5):1-9,9.基金项目
福建省中青年教师教育科研项目(JAT220070) （JAT220070）
福建省科技计划项目[高校产学合作项目(2023N5004),福建省星火项目(2023S0007,2023S0015,2023S0054),福建省对外合作项目(2023I1009)] （2023N5004）
福建农林大学农村振兴服务团队-草食性动物产业服务团队项目(11899170139) （11899170139）
