湖北科技学院学报（医学版）2026，Vol.40Issue(2)：93-98,6.DOI:10.16751/j.cnki.2095-4646.2025101706
基于Nrf2/HO-1通路探讨反-查耳酮对肠上皮细胞铜死亡的影响
Effects of Trans-Chalcone on Cuproptosis in Intestinal Epithelial Cells Via the Nrf2/HO-1 Signaling Pathway
摘要
Abstract
Objective To investigate the effects of trans-chalcone on cuproptosis in intestinal epithelial cells,focusing on the Nrf2/HO-1 signaling pathway.Methods An in vitro ulcerative colitis(UC)-associated cuproptosis model was established by treating NCM460 cells with 30 mg/mL dextran sulfate sodium(DSS)plus copper chloride(CuCl2).After 24 h of trans-chalcone treatment,cell viability was assessed via Cell Counting Kit-8(CCK-8);intracellular copper concentration,glutathione(GSH)levels,and reactive oxygen species(ROS)production were measured using respective assay kits.Inflammatory cytokines(TNF-α,IL-1 β,IL-6)were quantified by enzyme-linked immunosorbent assay(ELISA).Western blot analysis was used to detect protein expression of cuproptosis markers(FDX1,LIAS)and Nrf2/HO-1 signaling pathway-related proteins.Results Treatment of NCM460 cells with 30 mg/mL DSS plus 30 μmol/L CuCl2 signifi-cantly reduced cell viability,accompanied by marked alterations in cuproptosis-related indicators(intracellular copper concentration,GSH levels,and ROS production),confirming successfully establishing an inflammatory cell cuproptosis model.Notably,30 μmol/L trans-chal-cone effectively restored DSS+CuCl2-induced reduction in cell viability,effectively inhibited the secretion of inflammatory cytokines,de-creased intracellular copper ions and ROS levels,and enhanced GSH levels.Furthermore,trans-chalcone modulated the protein expression of cuproptosis markers(FDX1,LIAS)and key proteins in the Nrf2/HO-1 signaling pathway induced by DSS+CuCl2(all P＜0.05).Conclu-sion Trans-chalcone may alleviate DSS and CuCl2-induced cuproptosis in UC-associated intestinal epithelial cells by regulating the Nrf2/HO-1 signaling pathway.关键词
反-查耳酮/溃疡性结肠炎/肠上皮细胞/铜死亡/Nrf2/HO-1信号通路Key words
Trans-chalcone/Ulcerative colitis/Intestinal epithelial cells/Cuproptosis/Nrf2/HO-1 signaling pathway分类
医药卫生引用本文复制引用
翟祥洁,王寒,涂毅威,王森,李荣杰,汪慧,朱薿..基于Nrf2/HO-1通路探讨反-查耳酮对肠上皮细胞铜死亡的影响[J].湖北科技学院学报（医学版）,2026,40(2):93-98,6.基金项目
湖北省科技厅面上项目(2023AFB1120) （2023AFB1120）
湖北省教育厅科学研究计划项目(D20242804) （D20242804）
湖北科技学院校内科研发展基金项目医学部专项(2023YKY07) （2023YKY07）
口腔与眼视光医学院专项(2021WG02) （2021WG02）
