刑事技术2026，Vol.51Issue(2)：142-148,7.DOI:10.16467/j.1008-3650.2025.0002
UAIL-DLLME-LC-MS/MS测定血液中地芬尼多与苯海拉明
Determination of Difenidol and Diphenhydramine in Blood Samples by Ultrasonic-assisted Ionic Liquid-based Dispersive Liquid-liquid Microextraction Coupled with LC-MS/MS
摘要
Abstract
A method for the determination of difenidol and diphenhydramine in blood samples utilizing ultrasonic-assisted ionic liquid dispersive liquid-liquid microextraction coupled with LC-MS/MS was established.l-butyl-3-methylimidazolium hexafluorophosphate([Bmim][PF6])was used as the extractant,and acetone was used as the dispersant.The samples were separated by a ZORBAX Eclipse Plus C18 RRHD column.Aqueous solution containing 0.1％formic acid and methanol was used as mobile phases.A gradient elution mode was employed with a flow rate of 0.2 mL/min,followed by electrospray ion source mode(ESI+)analysis in scheduled multiple reaction monitoring mode(MRM).The detection limits of difenidol and diphenhydramine in blood samples were 0.01 ng/mL,and the quantification limits were 0.02 ng/mL for both.The intraday and interday precisions(relative standard deviation,RSD)were both less than 20％,and the relative recovery rates ranged from 98.38％to 115.10％.The concentrations of difenidol and diphenhydramine in the blood of a fatal case were 4.02 μg/mL and 3.78 μg/mL,respectively.This study demonstrated the feasibility of using ultrasonic-assisted ionic liquid dispersive liquid-liquid microextraction combined with LC-MS/MS for the determination of difenidol and diphenhydramine in blood samples.The method has the advantages of environmentally friendly,high enrichment efficiency,and high sensitivity,making it suitable for forensic identification of difenidol and diphenhydramine.关键词
法医毒物分析/超声辅助离子液体-分散液液微萃取/液相色谱-串联质谱/地芬尼多/苯海拉明Key words
forensic toxicological analysis/ultrasonic-assisted ionic liquid dispersive liquid-liquid microextraction(UAIL-DLLME)/LC-MS/MS/difenidol/diphenhydramine分类
社会科学引用本文复制引用
师燕华,孙赟,郭月,管青林,王涛,胡萌,尉志文,贠克明,崔海燕..UAIL-DLLME-LC-MS/MS测定血液中地芬尼多与苯海拉明[J].刑事技术,2026,51(2):142-148,7.基金项目
国家重点研发计划(2017YFC0803504) （2017YFC0803504）
国家青年科学基金项目(81601647) （81601647）
法医病理学公安部重点实验室2023年度开放课题(GAFYBL202304) （GAFYBL202304）
山西省教育厅高校科技创新项目(2020L0191) （2020L0191）
