烟草科技2026，Vol.59Issue(4)：49-57,9.DOI:10.16135/j.issn1002-0861.2026.0028
烟草靶斑病拮抗菌株CZ-56的鉴定及生防与促生作用
Identification,biocontrol and growth-promoting effects of an antagonistic strain CZ-56 against tobacco target spot
摘要
Abstract
To screen rhizosphere growth-promoting bacteria with good control effect on tobacco target spot,antagonistic bacteria against pathogen of tobacco target spot were obtained from rhizosphere soil of healthy tobacco plants by plate isolation culture and confrontation culture.The strain with the best antagonistic effects was then identified by morphological,biochemical and physiological characteristics and 16S rDNA phylogenetic analysis.The growth-promoting and resistance induction effects of the antagonistic strain on tobacco plants and its control efficiency on tobacco target spot were studied.The results showed that an R.solani.In vitro test confirmed that strain CZ-56 could effectively prevent the expansion of tobacco target spot.The preventive and therapeutic efficiencies in pot experiments were 72.58％and 83.06％,respectively.The fermentation filtrate of CZ-56 also promoted the growth of tobacco seedlings and increased the activity of defense enzymes,including polyphenol oxidase(PPO),superoxide dismutase(SOD),peroxidase(POD)and phenylalanine ammonlyase(PAL)in tobacco leaves.Therefore,strain CZ-56 has good control effects on tobacco target spot.关键词
烟草靶斑病/拮抗菌/摩氏假单胞菌/促生作用/防治效果/诱导抗性Key words
Tobacco target spot/Antagonistic bacteria/Pseudomonas mosselii/Growth-promoting effect/Control efficiency/Induced resistance分类
农业科技引用本文复制引用
尹秀娟,郭蕊,王迎,周向平,陈颐,刘天波,钟杰,肖艳松..烟草靶斑病拮抗菌株CZ-56的鉴定及生防与促生作用[J].烟草科技,2026,59(4):49-57,9.基金项目
湖南省郴州市烟草公司烟草农业科技创新项目"郴州烟区烟草靶斑病和野火病病原微生物组解析及早期流行诊断"(CZYC2022JS03)、"烟草镰刀根腐病的靶向防控技术研究与应用"(CZYC2024JS05). （CZYC2022JS03）
