烟草科技2026，Vol.59Issue(4)：70-80,112,12.DOI:10.16135/j.issn1002-0861.2025.0466
SA-GE复合凝胶珠固定化果胶酶对烟草提取物品质的影响
Effects of pectinase immobilizd by SA-GE composite gel beads on tobacco extract quality
摘要
Abstract
Tobacco extracts are important tobacco flavors,but macromolecular impurities such as pectin can have adverse effects on the sensory quality.To reduce pectin content and to improve quality and aroma of tobacco extracts,a composite carrier of gel beads with sodium alginate-gelatin(SA-GE)were built to immobilize pectinase.By conducting single-factor experiments and the response surface optimization,the preparation process of pectinase immobilization was optimized and its application effects in tobacco extracts were evaluated.The results showed that:1)When the total content of SA and GE was 4%and m(SA)∶m(GE)=1.0∶1.0,the immobilization capacity of pectinase was high,and the enzyme activity recovery rate of pectinase was the highest.Under the conditions of an initial pectinase content of 3%,an immobilization temperature of 33℃,an immobilization pH value of 3.6,and an immobilization time of 3.5 h,the enzyme activity recovery rate reached 46.99%.2)Compared with free pectinase,the immobilized pectinase maintained a high relative enzyme activity when both the contents of ethanol and propylene glycol were 50%.When the pH value was 1-7,the stability of immobilized pectinase was relatively high.When the temperature was 80℃,the relative enzyme activity of immobilized pectinase was 63.47%.After 5 repeated uses,the relative enzyme activity still exceeded 40%.3)After adding Yuyan extract treated with immobilized pectinase to cigarettes,the cigarette samples exhibited enhanced sensory performance with increased aroma richness,reduced off-notes,and significantly diminished irritation.This study demonstrates the feasibility of improving the quality of tobacco extracts through SA-GE composite gel immobilization technology,providing a new pathway for enhancing the quality and efficiency of tobacco extracts.关键词
烟草提取物/固定化/果胶酶/海藻酸钠/明胶Key words
Tobacco extract/Immobilization/Pectinase/Sodium alginate/Gelatin分类
化学化工引用本文复制引用
刘丹,杨晓,张天雅,刘丹丹,黄阔,叶长文,王鲁峰..SA-GE复合凝胶珠固定化果胶酶对烟草提取物品质的影响[J].烟草科技,2026,59(4):70-80,112,12.基金项目
中国烟草总公司科技项目"烟草专用蛋白酶和淀粉酶的高效创制及应用研究"(110202102019). （110202102019）
