烟台大学学报（自然科学与工程版）2026，Vol.39Issue(2)：143-150,8.DOI:10.13951/j.cnki.37-1213/n.241201
基于pH变性及圆二色谱检测的PC645空间结构状态
PC645 Spatial Structure State Based on pH Denaturation and Circular Dichroism Detection
摘要
Abstract
By monitoring absorption spectra and circular dichroism(CD)spectra across different pH conditions,we investigated the stability of phycocyanin PC645's chromophore and spatial structure,along with its energy transfer function.The results demonstrated that:1)PC645 maintained stable absorption peaks,CD peaks,and secondary structure ratios with pH 3.5-10,with optimal protein conformation and functional state observed at pH 5-7.Nota-bly,pH 5 appeared to represent a critical transition point for physiological functional changes;2)Moderate struc-tural alterations occurred at extreme pH values(pH 3-3.5 and pH 10-11),where secondary structure changes were observed while tertiary and quaternary structures and energy absorption functions remained intact,suggesting the latter play a more crucial role in light harvesting.The 525 nm CD peak emerged as a reliable indicator of qua-ternary structural integrity;3)Significant structural disintegration occurred at pH<3 and pH>10,with acid denat-uration inducing secondary structure folding while alkaline conditions caused irregular peptide chain coiling.These findings highlight PC645's remarkable pH adaptability,suggesting its strong potential as a versatile fluorescent probe capable of withstanding diverse chemical environments for various fluorescence detection applications.关键词
蓝隐藻/PC645/pH稳定性/CD谱/空间结构状态Key words
Chroomonas placiodea/PC645/pH stability/circular dichroism/spatial structure state分类
农业科技引用本文复制引用
李晓彤,任靖文,赵明日,陈敏..基于pH变性及圆二色谱检测的PC645空间结构状态[J].烟台大学学报（自然科学与工程版）,2026,39(2):143-150,8.基金项目
国家自然科学基金资助项目(SM19N14). （SM19N14）
