空军军医大学学报2026，Vol.47Issue(4)：565-570,6.DOI:10.13276/j.issn.2097-1656.2026.04.014
二乙基亚硝胺诱导不同发育阶段小鼠原发性肝癌模型比较
Comparison of diethylnitrosamine-induced primary liver cancer models in mice at different developmental stages
摘要
Abstract
Objective To systematically compare the differences in tumorigenic efficiency,pathological characteristics,and molecular phenotypes between neonatal(14 d post-birth)and adult(7 weeks old)C57BL/6 mouse models of diethylnitrosamine-induced hepatocellular carcinoma(HCC).Methods Thirty wild-type C57BL/6 mice were selected and divided into a normal group,a neonatal group,and an adult group,with 10 mice in each group.The induced HCC mouse model was established by intraperitoneal injection of carbon tetrachloride combined with a high-fat diet.The survival rates,liver histomorphology,serum liver function indicators[alpha-fetoprotein(AFP),alanine aminotransferase,aspartate aminotransferase,total bilirubin,direct bilirubin,albumin,and triglyceride],pathological staining(HE,PAS,and ORO),and immunohistochemical staining were dynamically monitored to observe the expression and distribution of Ki67 and AFP.Results The pathological features of liver tissues and serum AFP levels(P<0.01)in the neonatal group more closely resembled the clinical progression pattern of HCC.In contrast,the adult group exhibited a prolonged latency period and milder metabolic disturbances.Conclusion This study provides critical experimental evidence for the selection of induced HCC animal models and advances research on developmental stage-dependent carcinogenic mechanisms.关键词
肝肿瘤/二乙基亚硝胺/疾病模型,动物/年龄因素/癌发生/免疫组织化学/饮食,高脂/肿瘤分级Key words
liver neoplasms/diethylnitrosamine/disease models,animal/age factors/carcinogenesis/immunohistochemistry/diet,high-fat/neoplasm grading分类
医药卫生引用本文复制引用
史倩倩,茹梦瑶,白雨,张坤,郭燕,白占涛,沈岚..二乙基亚硝胺诱导不同发育阶段小鼠原发性肝癌模型比较[J].空军军医大学学报,2026,47(4):565-570,6.基金项目
国家自然科学基金面上项目(81672542) （81672542）
陕西省自然科学基础研究计划重点项目(2021JZ-27) （2021JZ-27）
消化系肿瘤整合防治全国重点实验室开放课题(CBSKL2022ZDKF12) （CBSKL2022ZDKF12）
消化系肿瘤整合防治全国重点实验室自主课题(2025GTEP010) （2025GTEP010）
空军军医大学自主课题(2022-fhjsyxrc08) （2022-fhjsyxrc08）
