医药导报2026，Vol.45Issue(5)：862-869,8.DOI:10.3870/j.issn.1004-0781.2026.05.019
低共熔溶剂提取绞股蓝黄酮类成分的工艺优选
Optimization of Deep Eutectic Solvent Extraction Process of Flavonoids from Gynostemma pentaphyllum
摘要
Abstract
Objective To optimize the extraction process of rutin,quercetin,and kaempferol,three major flavonoid components,from Gynostemma pentaphyllum.Methods A HPLC method was used to analyze the content.The total extraction yield of three flavonoid components-rutin,quercetin,and kaempferol-was selected as the evaluation index.The optimal deep eutec-tic solvent system was determined by examining the type,molar ratio,and water content of the deep eutectic solvent.Box-Behnken response surface methodology was used to investigate the effects of liquid-solid ratio,ultrasonic temperature,and ultrasonic time on the extraction of Gynostemma pentaphyllum flavonoid components to determine the optimal extraction conditions.Results The optimum extraction process of three flavonoid components from Gynostemma pentaphyllum was as follows:the solvent composition consists of choline chloride and oxalic acid at a molar ratio of 3∶1,with a water content of 30%.Additionally,the liquid-to-solid ratio is maintained at 35 mL·g-1,the ultrasonic temperature is set at 69℃,and the ultrasonic duration is 40 minutes.Conclu-sion The optimized deep eutectic solvent-based extraction method demonstrates stability and feasibility,providing a reliable ap-proach for the efficient and safe extraction of flavonoids from Gynostemma pentaphyllum.关键词
绞股蓝/黄酮/低共熔溶剂/响应曲面法/提取工艺Key words
Gynostemma pentaphyllum/Flavonoids/Deep eutectic solvent/Response surface method/Extraction techno-logy分类
医药卫生引用本文复制引用
王雪芹,陈娜娜,陈书谚,吕光辉,黄良永,叶方..低共熔溶剂提取绞股蓝黄酮类成分的工艺优选[J].医药导报,2026,45(5):862-869,8.基金项目
2024年度十堰市引导性科研项目(24Y035). （24Y035）
