应用化学2026，Vol.43Issue(4)：544-553,10.DOI:10.19894/j.issn.1000-0518.250333
用于快速止血湿粘附性的壳聚糖基复合水凝胶
Wet Adhesive Chitosan-Based Composite Hydrogel for Rapid Hemostasis
摘要
Abstract
A lactose-modified chitosan(CTL)/4-arm polyethylene glycol succinate(4-arm-PEG-NHS)hydrogel(CPN)was meticulously formulated on the basis of amide bonds,utilizing a triad of key constituents:chitosan,lactose and 4-arm-PEG-NHS.The experimental results demonstrated that the CPN hydrogel was capable of rapidly establishing robust covalent bonds with pig skin(a representative of biological tissue)and firmly adhering to its surface.The viability of mouse fibroblast cells(L929)treated with CPN hydrogel extract for 1,3 and 5 days was greater than 90％.Furthermore,the calcein acetoxymethyl ester/propidium iodide(calcein-AM/PI)double staining test also demonstrated that the CPN hydrogel exhibited excellent compatibility with cells.The hemolysis rate of CPN hydrogel dispersion was less than 5％at all test concentrations,indicating excellent hemocompatibility of the CPN hydrogel.In addition,the blood coagulation index(BCI)can reach 18.9％after 10 min in the coagulation test in vitro.In the Sprague-Dawley rat liver hemostasis model,the untreated rats required a mean of(145.6±29.7)s to achieve hemostasis,whereas those treated with CPN hydrogel required a mean of only(33.9±17.0)s.The bleeding loss of the untreated rats was(734.8±369.6)mg,whereas those of the rats treated with CPN hydrogel were significantly reduced to(71.2±20.2)mg.The results showed that the CPN hydrogel demonstrated excellent hemostatic properties,suggesting its potential application in hemostatic materials.关键词
壳聚糖/N-羟基琥珀酰亚胺酯/止血水凝胶/生物粘附性Key words
Chitosan/N-Hydroxy succinimide ester/Hemostatic hydrogel/Bio-adhesiveness分类
化学化工引用本文复制引用
刘雨,朱萌,岳乐,田野,牛忠伟..用于快速止血湿粘附性的壳聚糖基复合水凝胶[J].应用化学,2026,43(4):544-553,10.基金项目
国家自然科学基金(Nos.52073293,82204326,52273160)和理化技术研究所所长基金资助 Supported by the National Natural Science Foundation of China(Nos.52073293,82204326,52273160)and the Director's Fund of Technology Institute of Physical and Chemical （Nos.52073293,82204326,52273160）
