园艺学报2026，Vol.53Issue(3)：697-708,12.DOI:10.16420/j.issn.0513-353x.2025-0102
低温胁迫下亚精胺对菜豆幼苗PSⅡ功能及叶绿素代谢关键基因表达的影响
Effects of Spermidine on PSⅡ Function and Expression of Key Genes of Chlorophyll Metabolism in Common Bean Seedlings Under Low Temperature Stress
摘要
Abstract
In this study,the effects of 1.0 mmol·L-1 exogenous spermidine(Spd)and methylglyoxal diamidine hydrazone(MGBG)on the growth,chlorophyll content,photosystemⅡ(PSⅡ)function,chlorophyll degradation and synthase activity and related gene expression of common bean seedlings under low temperature stress were investigated at 12℃/8℃.The results indicated that compared with the control,low-temperature stress inhibited the growth of seedlings;chlorophyll content maximum photochemical efficiency of PSⅡ(Fv/Fm),photochemical quenching coefficient(qP),electron transport rate(ETR)and actual photochemical efficiency(ΦPSII)were all significantly reduced(P<0.05);while non-photochemical quenching coefficient(qN)significantly increased(P<0.05).Under low-temperature stress,the chlorophyll fluorescence induction(OJIP)curve showed significant alterations:the fluorescence intensities at the J-and P-steps were reduced,while the heat dissipation per unit cross-section(DIo/CSm)and energy dissipation per reaction center(DIo/RC)were markedly elevated.Under low temperatures(T2),the application of Spd reversed the aforementioned trends,with significant increases in light energy absorption(ABS/CSm),conversion(TRo/CSm),and electron transport(ETo/CSm).Spd-induced downregulation of key chlorophyll degradation genes PvCLH1(chlorophyllase 1)/PvCLH2(chlorophyllase 2)and PvRCCR1(red chlorophyll metabolite reductase 1)resulted in decreased chlorophyllase(CLH)activity;Key chlorophyll synthesis genes PvPOR1(proto-deplastidic chlorophyll reductase 1)/PvPOR2(proto-deplastidic chlorophyll reductase 2)showed significantly increased relative expression(P<0.05),with elevated chlorophyll acidase activity.The results indicate that exogenous Spd alleviates the suppression of photosynthesis in common bean seedlings under low-temperature stress by upregulating the expression of chlorophyll synthesis-related genes,increasing chlorophyll content,and enhancing the absorption and utilization of light energy by PSⅡ.关键词
菜豆/低温胁迫/叶绿素代谢/亚精胺/PSⅡ/叶绿素荧光动力学曲线(OJIP)Key words
common bean/low temperature stress/chlorophyll metabolism/spermidine/PSⅡ/chlorophyll fluorescence kinetics curve(OJIP)分类
农业科技引用本文复制引用
曹金萍,杨文欢,刘秀娟,王玉萍..低温胁迫下亚精胺对菜豆幼苗PSⅡ功能及叶绿素代谢关键基因表达的影响[J].园艺学报,2026,53(3):697-708,12.基金项目
甘肃省重点研发项目(25YFNA035) （25YFNA035）
国家自然科学基金项目(31760351) （31760351）
