湖北医药学院学报2026，Vol.45Issue(2)：139-145,7.DOI:10.13819/j.issn.2096-708X.2026.02.003
血清抗dsDNA抗体联合IL-21、BCL6、CD71 mRNA水平对早期系统性红斑狼疮的诊断价值
Diagnostic Value of Serum Anti-dsDNA Antibodies Combined with IL-21,BCL6,and CD71 mRNA Expression in Peripheral Blood Mononuclear Cells for Early Systemic Lupus Erythematosus
摘要
Abstract
Objective To investigate the clinical diagnostic value of anti-double-stranded(ds)DNA antibody combined with interleukin-21(IL-21),B-cell lymphoma 6(BCL6),and transferrin receptor(CD71)mRNA levels for early sys-temic lupus erythematosus(SLE).Methods Blood samples were collected from 41 newly diagnosed SLE patients(inclu-ding 8 in the active phase and 33 in the inactive phase)and 41 healthy controls between January 2021 and June 2025.Pe-ripheral blood mononuclear cells(PBMCs)were isolated,and the mRNA levels of IL-21,BCL6,and CD71 were meas-ured.Serum anti-dsDNA antibodies were detected by immunoblotting.Spearman correlation analysis was used to assess the relationship between the expression of the markers and the SLE disease activity index(SLEDAI)scores.Receiver operating characteristic(ROC)curve analysis was performed to evaluate the diagnostic efficacy of anti-dsDNA antibody combined with IL-21,BCL6,and CD71 gene expression for SLE.Results The levels of IL-21,BCL6,and CD71 in PBMCs were higher in SLE patients than in healthy controls(P＜0.05),and their expressions were elevated in active SLE patients com-pared to inactive patients(P＜0.0001).Spearman correlation analysis indicated that the expressions of these markers were positively correlated with SLEDAI scores(P＜0.01).The combination of anti-dsDNA antibodies and IL-21,BCL6,and CD71 genes achieved an area under the curve(AUC)of 0.952,with a sensitivity of 92.7％and specificity of 87.8％.Con-clusion The expressions of IL-21,BCL6,and CD71 genes are significantly elevated in SLE patients and positively corre-late with SLEDAI scores.Anti-dsDNA antibodies along with IL-21,BCL6,and CD71 gene expressions provide excellent diagnostic performance for early SLE.关键词
系统性红斑狼疮/B细胞淋巴瘤因子6/白细胞介素-21/转铁蛋白受体1Key words
Systemic lupus erythematosus/BCL6/IL-21/CD71引用本文复制引用
邢紫依,李静,赵卫卫..血清抗dsDNA抗体联合IL-21、BCL6、CD71 mRNA水平对早期系统性红斑狼疮的诊断价值[J].湖北医药学院学报,2026,45(2):139-145,7.基金项目
上海市奉贤区科学技术委员会课题(20211609) （20211609）
