湖北医药学院学报2026，Vol.45Issue(2)：164-172,9.DOI:10.13819/j.issn.2096-708X.2026.02.007
响应面法优化大孔树脂纯化毛连菜中有机酸的工艺研究
Optimization of the Purification Process for Organic Acids from Picris hieracioides L.Using Macroporous Resin by Response Surface Methodology
摘要
Abstract
Objective To optimize the purification process for organic acids from Picris hieracioides L.using macroporous resin by the Box-Behnken response surface methodology based on single-factor experiments.Methods Organic acids(pri-marily chlorogenic acid)extracted from P.hieracioides using deep eutectic solvents were selected.The optimal macroporous resin was screened by comparing the adsorption and desorption capacities of seven resins through static experiments.The effects of aspect ratio,eluent concentration,and elution volume on the purification were investigated using single-factor ex-periments.Subsequently,response surface methodology was employed to optimize these three critical parameters to deter-mine the optimal purification conditions.Results The NKA-2 macroporous resin was identified as the most suitable for pu-rifying organic acids.The optimal purification conditions were an aspect ratio of 8∶1,an eluent concentration of 80％,and an elution volume of 3 BV.Under these conditions,the total recovery rate of organic acids from P.hieracioides reached 90.38％,with a relative error of 0.64％compared to the predicted value.Conclusion The NKA-2 resin demonstrated ex-cellent performance in purifying organic acids from P.Hieracioides.The optimized process is stable and feasible,showing potential for industrial application.关键词
毛连菜/绿原酸/低共熔溶剂/响应面法/大孔树脂/纯化工艺Key words
Picris hieracioides L./Chlorogenic acid/Deep eutectic solvent/Response surface methodology/Macroporous resin/Purification process引用本文复制引用
吕光辉,何亮,叶旭雯,王雪芹,罗迎菲,李燕,叶方..响应面法优化大孔树脂纯化毛连菜中有机酸的工艺研究[J].湖北医药学院学报,2026,45(2):164-172,9.基金项目
十堰市市级引导性科研项目(21Y03,24Y035) （21Y03,24Y035）
湖北医药学院"十四五"省级优势特色学科群(生物与医药)资助项目(2022BMXKQT3) （生物与医药）
