中草药2026，Vol.57Issue(9)：3300-3312,13.DOI:10.7501/j.issn.0253-2670.2026.09.004
基于代谢组学的不同山药种质资源的化学比较
Chemical comparison of different Dioscoreae Rhizoma germplasm resources based on metabolomics
摘要
Abstract
Objective To compare different varieties of Dioscoreae Rhizoma and reveal their chemical differences,providing a basis for targeted breeding of Dioscoreae Rhizoma varieties.Methods Eight batches of Dioscoreae Rhizoma materials with different germplasms were collected,their agronomic traits were measured and compared.Subsequently,ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS)-based untargeted metabolomic approach was employed to systematically analyze their chemical compositions.Multivariate statistical methods,including principal component analysis(PCA),cluster heatmap analysis and correlation plots,were used to dissect the metabolic product differences among varieties and their associations with agronomic traits.Results The eight batches of Dioscoreae Rhizoma showed significant differences in indicators such as yield and tuber morphology.Based on high-resolution mass spectrometry,164 chemical components were identified across the eight batches of Dioscoreae Rhizoma,including peptides,amino acids,flavonoids,and saponins.PCA indicated significant differences among the eight batches of Dioscoreae Rhizoma germplasm resources.Saponin levels were relatively high in Jin Dioscoreae Rhizoma,Taigu Dioscoreae Rhizoma,Shuangbang Dioscoreae Rhizoma,and Ma Dioscoreae Rhizoma,while Taigu Dioscoreae Rhizoma,Jin Dioscoreae Rhizoma,and Ma Dioscoreae Rhizoma exhibited similar chemical characteristics in terms of flavonoids.Cluster heatmap analysis revealed potential chemical markers,providing a basis for rapid discrimination among different varieties.The chemical differences between medicinal-edible Dioscoreae Rhizoma varieties such as Tiegun Dioscoreae Rhizoma and Shandong Ximao and other varieties may be related with amino acids and peptides.The correlation network indicated that stem diameter was significantly positively correlated with saponins,while bulbils weight showed positive correlations with saponins and flavonoids but negative correlations with amino acids and peptides.Conclusion The eight different batches of Dioscoreae Rhizoma possess distinct chemical characteristics,which provide a scientific basis for the targeted breeding of medicinal and edible-specific Dioscoreae Rhizoma varieties.关键词
山药/薯蓣/代谢组学/UPLC-Q-TOF-MS/MS/种质资源/化学比较Key words
Dioscoreae Rhizoma/Dioscorea opposita Thunb./metabolomics/UPLC-Q-TOF-MS/MS/germplasm resources/chemical comparisons分类
医药卫生引用本文复制引用
王晓民,胡晓丽,许爱玲,关望辉,韩丽丽,张鹏飞,李震宇..基于代谢组学的不同山药种质资源的化学比较[J].中草药,2026,57(9):3300-3312,13.基金项目
国家农业农村部现代农业产业技术体系山药运城综合试验站(CARS-21) （CARS-21）
山药种质资源创制与新品种选育研究(YCKJ-2023014) （YCKJ-2023014）
国家中医药管理局青年岐黄学者培养项目(2022-256) （2022-256）
